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dc.contributor.authorOrsi, William D.  Concept link
dc.contributor.authorEdgcomb, Virginia P.  Concept link
dc.contributor.authorChristman, Glenn D.  Concept link
dc.contributor.authorBiddle, Jennifer F.  Concept link
dc.date.accessioned2013-06-28T18:11:10Z
dc.date.available2014-10-22T08:57:22Z
dc.date.issued2013-04-23
dc.identifier.urihttps://hdl.handle.net/1912/6024
dc.descriptionAuthor Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature 499 (2013): 205-208, doi:10.1038/nature12230.en_US
dc.description.abstractScientific ocean drilling has revealed a deep biosphere of widespread microbial life in sub-seafloor sediment. Microbial metabolism in the marine subsurface likely plays an important role in global biogeochemical cycles1-3 but deep biosphere activities are not well understood1. Here, we describe and analyze the first subseafloor metatranscriptomes from anaerobic Peru Margin sediment up to 159 meters below seafloor (mbsf) represented by over 1 billion cDNA sequence reads. Anaerobic metabolism of amino acids, carbohydrates, and lipids appear to be dominant metabolic processes, and profiles of dissimilatory sulfite reductase (Dsr) transcripts are consistent with porewater sulfate concentration profiles1. Moreover, transcripts involved in cell division increase as a function of microbial cell concentration, indicating that increases in subseafloor microbial abundance are a function of cell division across all three domains of life. These data support calculations1 and models4 of subseafloor microbial metabolism and represent the first holistic picture of deep biosphere activities.en_US
dc.description.sponsorshipThis work was fostered by a Center for Dark Energy Biosphere Investigations (CDEBI) grant OCE-0939564 to WO and a NSF IOS grant 1238801 to JFB.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1038/nature12230
dc.subjectMarine subsurfaceen_US
dc.subjectmRNAen_US
dc.subjectMetatranscriptomicsen_US
dc.subjectDeep biosphereen_US
dc.subjectIllumina sequencingen_US
dc.subjectMarine sedimenten_US
dc.subjectSubseaflooren_US
dc.subjectODP Leg 201en_US
dc.subjectGene expressionen_US
dc.titleGene expression in the deep biosphereen_US
dc.typePreprinten_US
dc.description.embargo2013-12-12en_US


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