Gene expression in the deep biosphere
Orsi, William D.
Edgcomb, Virginia P.
Christman, Glenn D.
Biddle, Jennifer F.
MetadataShow full item record
KeywordMarine subsurface; mRNA; Metatranscriptomics; Deep biosphere; Illumina sequencing; Marine sediment; Subseafloor; ODP Leg 201; Gene expression
Scientific ocean drilling has revealed a deep biosphere of widespread microbial life in sub-seafloor sediment. Microbial metabolism in the marine subsurface likely plays an important role in global biogeochemical cycles1-3 but deep biosphere activities are not well understood1. Here, we describe and analyze the first subseafloor metatranscriptomes from anaerobic Peru Margin sediment up to 159 meters below seafloor (mbsf) represented by over 1 billion cDNA sequence reads. Anaerobic metabolism of amino acids, carbohydrates, and lipids appear to be dominant metabolic processes, and profiles of dissimilatory sulfite reductase (Dsr) transcripts are consistent with porewater sulfate concentration profiles1. Moreover, transcripts involved in cell division increase as a function of microbial cell concentration, indicating that increases in subseafloor microbial abundance are a function of cell division across all three domains of life. These data support calculations1 and models4 of subseafloor microbial metabolism and represent the first holistic picture of deep biosphere activities.
Author Posting. © The Author(s), 2013. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature 499 (2013): 205-208, doi:10.1038/nature12230.
Suggested CitationPreprint: Orsi, William D., Edgcomb, Virginia P., Christman, Glenn D., Biddle, Jennifer F., "Gene expression in the deep biosphere", 2013-04-23, https://doi.org/10.1038/nature12230, https://hdl.handle.net/1912/6024
Showing items related by title, author, creator and subject.
Dewar, William K.; Bingham, Rory J.; Iverson, R. L.; Nowacek, Douglas P.; St. Laurent, Louis C.; Wiebe, Peter H. (Sears Foundation for Marine Research, 2006-07)Ocean mixing is thought to control the climatically important oceanic overturning circulation. Here we argue the marine biosphere, by a mechanism like the bioturbation occurring in marine sediments, mixes the oceans as ...
Salt marsh sediment diversity : a test of the variability of the rare biosphere among environmental replicates Bowen, Jennifer L.; Morrison, Hilary G.; Hobbie, John E.; Sogin, Mitchell L. (2011-12-17)Much of the phylogenetic diversity in microbial systems arises from rare taxa that comprise the long tail of taxon rank distribution curves. This vast diversity presents a challenge to testing hypotheses about the effects ...
Mapping the biosphere : exploring species to understand the origin, organization and sustainability of biodiversity Wheeler, Q. D.; Knapp, Sandra; Stevenson, D. W.; Stevenson, J.; Blum, Stan D.; Boom, B.. M.; Borisy, Gary G.; Buizer, James L.; De Carvalho, M. R.; Cibrian, A.; Donoghue, M. J.; Doyle, V.; Gerson, E. M.; Graham, C. H.; Graves, P.; Graves, Sara J.; Guralnick, Robert P.; Hamilton, A. L.; Hanken, J.; Law, W.; Lipscomb, D. L.; Lovejoy, T. E.; Miller, Holly; Miller, J. S.; Naeem, Shahid; Novacek, M. J.; Page, L. M.; Platnick, N. I.; Porter-Morgan, H.; Raven, Peter H.; Solis, M. A.; Valdecasas, A. G.; Van Der Leeuw, S.; Vasco, A.; Vermeulen, N.; Vogel, J.; Walls, R. L.; Wilson, E. O.; Woolley, J. B. (Taylor & Francis, 2012-03-27)The time is ripe for a comprehensive mission to explore and document Earth's species. This calls for a campaign to educate and inspire the next generation of professional and citizen species explorers, investments in ...