Now showing items 1-20 of 196

  • Accuracy and quality of massively parallel DNA pyrosequencing 

    Huse, Susan M.; Huber, Julie A.; Morrison, Hilary G.; Sogin, Mitchell L.; Mark Welch, David B. (BioMed Central, 2007-07-20)
    Massively parallel pyrosequencing systems have increased the efficiency of DNA sequencing, although the published per-base accuracy of a Roche GS20 is only 96%. In genome projects, highly redundant consensus assemblies can ...
  • Adaptive evolution of voltage-gated sodium channels : the first 800 million years 

    Zakon, Harold H. (2012-04)
    Voltage-gated Na+-permeable (Nav) channels form the basis for electrical excitability in animals. Nav channels evolved from Ca2+ channels and were present in the common ancestor of choanoflagellates and animals although ...
  • Alkenone producers inferred from well-preserved 18S rDNA in Greenland lake sediments 

    D'Andrea, William J.; Lage, Melissa; Martiny, Jennifer B. H.; Laatsch, Abby D.; Amaral-Zettler, Linda A.; Sogin, Mitchell L.; Huang, Yongsong (American Geophysical Union, 2006-09-19)
    The 18S ribosomal DNA (rDNA) sequences of haptophyte algae were successfully amplified using the polymerase chain reaction (PCR) from water filtrate, surface sediments, and a late-Holocene sediment sample (∼1000 years old) ...
  • Analysis of expressed sequence tags of the cyclically parthenogenetic rotifer Brachionus plicatilis 

    Suga, Koushirou; Mark Welch, David B.; Tanaka, Yukari; Sakakura, Yoshitaka; Hagiwara, Atsushi (Public Library of Science, 2007-08-01)
    Rotifers are among the most common non-arthropod animals and are the most experimentally tractable members of the basal assemblage of metazoan phyla known as Gnathifera. The monogonont rotifer Brachionus plicatilis is a ...
  • Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys 

    Nelson, Michael C.; Morrison, Hilary G.; Benjamino, Jacquelynn; Grim, Sharon L.; Graf, Joerg (Public Library of Science, 2014-04-10)
    The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 ...
  • Archaea and bacteria with surprising microdiversity show shifts in dominance over 1,000-year time scales in hydrothermal chimneys 

    Brazelton, William J.; Ludwig, Kristin A.; Sogin, Mitchell L.; Andreishcheva, Ekaterina N.; Kelley, Deborah S.; Shen, Chuan-Chou; Edwards, R. Lawrence; Baross, John A. (2009-12-06)
    The Lost City Hydrothermal Field, an ultramafic-hosted system located 15 km west of the Mid-Atlantic Ridge, has experienced at least 30,000 years of hydrothermal activity. Previous studies have shown that its carbonate ...
  • ASPIRE : the Amundsen Sea Polynya International Research Expedition 

    Yager, Patricia L.; Sherrell, Robert M.; Stammerjohn, Sharon E.; Alderkamp, Anne-Carlijn; Schofield, Oscar M. E.; Abrahamsen, E. Povl; Arrigo, Kevin R.; Bertilsson, Stefan; Garay, D. Lollie; Guerrero, Raul; Lowry, Kate E.; Moksnes, Per-Olav; Ndungu, Kuria; Post, Anton F.; Randall-Goodwin, Evan; Riemann, Lasse; Severmann, Silke; Thatje, Sven; van Dijken, Gert L.; Wilson, Stephanie (The Oceanography Society, 2012-09)
    In search of an explanation for some of the greenest waters ever seen in coastal Antarctica and their possible link to some of the fastest melting glaciers and declining summer sea ice, the Amundsen Sea Polynya International ...
  • Axial Seamount 

    Chadwick, William W.; Butterfield, David A.; Embley, Robert W.; Tunnicliffe, Verena; Huber, Julie A.; Nooner, Scott L.; Clague, David A. (Oceanography Society, 2010-03)
    Axial Seamount is a hotspot volcano superimposed on the Juan de Fuca Ridge (JdFR) in the Northeast Pacific Ocean. Due to its robust magma supply, it rises ~ 800 m above the rest of JdFR and has a large elongate summit ...
  • Bacterial group II introns in a deep-sea hydrothermal vent environment 

    Podar, Mircea; Mullineaux, Lauren S.; Huang, Hon-Ren; Perlman, Philip S.; Sogin, Mitchell L. (American Society for Microbiology, 2002-12)
    Group II introns are catalytic RNAs and mobile retrotransposable elements known to be present in the genomes of some nonmarine bacteria and eukaryotic organelles. Here we report the discovery of group II introns in a ...
  • Bidirectional incompatibility among divergent Wolbachia and incompatibility level differences among closely related Wolbachia in Nasonia 

    Bordenstein, Seth R.; Werren, John H. (2007-03-07)
    Most insect groups harbor obligate bacterial symbionts from the alphaproteobacterial genus Wolbachia. These bacteria alter insect reproduction in ways that enhance their cytoplasmic transmission. One of the most common ...
  • A bifunctional DNA binding region in Tn5 transposase 

    Gradman, Richard J.; Ptacin, Jerod L.; Bhasin, Archna; Reznikoff, William S.; Goryshin, Igor Y. (Blackwell, 2007-12-16)
    Tn5 transposition is a complicated process that requires the formation of a highly ordered protein–DNA structure, a synaptic complex, to catalyse the movement of a sequence of DNA (transposon) into a target DNA. Much is ...
  • Biogeography and ecology of the rare and abundant microbial lineages in deep-sea hydrothermal vents 

    Anderson, Rika E.; Sogin, Mitchell L.; Baross, John A. (2014-11)
    Environmental gradients generate countless ecological niches in deep-sea hydrothermal vent systems, which foster diverse microbial communities. The majority of distinct microbial lineages in these communities occur in ...
  • Broadly sampled multigene trees of eukaryotes 

    Yoon, Hwan Su; Grant, Jessica; Tekle, Yonas I.; Wu, Min; Chaon, Benjamin C.; Cole, Jeffrey C.; Logsdon, John M.; Patterson, David J.; Bhattacharya, Debashish; Katz, Laura A. (BioMed Central, 2008-01-18)
    Our understanding of the eukaryotic tree of life and the tremendous diversity of microbial eukaryotes is in flux as additional genes and diverse taxa are sampled for molecular analyses. Despite instability in many analyses, ...
  • The centrosome and spindle as a ribonucleoprotein complex 

    Alliegro, Mark C. (2011-01-03)
    The presence of nucleic acids in centrosomes and the spindle have been proposed, observed, and reported since the 1950s. Why did the subject remain, perhaps even until today, such a controversial issue? The explanation ...
  • Characterisation of the subtelomeric regions of Giardia lamblia genome isolate WBC6 

    Prabhu, Anjali; Morrison, Hilary G.; Martinez, Charles R.; Adam, Rodney D. (2006-12-04)
    Giardia trophozoites are polyploid and have five chromosomes. The chromosome homologues demonstrate considerable size heterogeneity due to variation in the subtelomeric regions. We used clones from the genome project with ...
  • Characterization of cyanate metabolism in marine Synechococcus and Prochlorococcus spp. 

    Kamennaya, Nina A.; Post, Anton F. (2010-10-14)
    Cyanobacteria of the genera Synechococcus and Prochlorococcus are the most abundant photosynthetic organism on Earth occupying a key position at the base of marine food webs. The cynS gene that encodes cyanase was identified ...
  • Chronic exposure to arsenic in the drinking water alters the expression of immune response genes in mouse lung 

    Kozul, Courtney D.; Hampton, Thomas H.; Davey, Jennifer C.; Gosse, Julie A.; Nomikos, Athena P.; Eisenhauer, Phillip L.; Weiss, Daniel J.; Thorpe, Jessica E.; Ihnat, Michael A.; Hamilton, Joshua W. (National Institute of Environmental Health Sciences, 2009-03-04)
    Chronic exposure to drinking water arsenic is a significant worldwide environmental health concern. Exposure to As is associated with an increased risk of lung disease, which may make it a unique toxicant, because lung ...
  • Ciliate diversity, community structure, and novel taxa in lakes of the McMurdo Dry Valleys, Antarctica 

    Xu, Yuan; Vick-Majors, Trista; Morgan-Kiss, Rachael; Priscu, John C.; Amaral-Zettler, Linda A. (Marine Biological Laboratory, 2014-10-01)
    We report an in-depth survey of next-generation DNA sequencing of ciliate diversity and community structure in two permanently ice-covered McMurdo Dry Valley lakes during the austral summer and autumn (November 2007 and ...
  • The coastal environment and human health : microbial indicators, pathogens, sentinels and reservoirs 

    Stewart, Jill R.; Gast, Rebecca J.; Fujioka, Roger S.; Solo-Gabriele, Helena M.; Meschke, J. Scott; Amaral-Zettler, Linda A.; del Castillo, Erika; Polz, Martin F.; Collier, Tracy K.; Strom, Mark S.; Sinigalliano, Christopher D.; Moeller, Peter D. R.; Holland, A. Fredrick (BioMed Central, 2008-11-07)
    Innovative research relating oceans and human health is advancing our understanding of disease-causing organisms in coastal ecosystems. Novel techniques are elucidating the loading, transport and fate of pathogens in coastal ...
  • Comparative proteome analysis of psychrophilic versus mesophilic bacterial species : insights into the molecular basis of cold adaptation of proteins 

    Metpally, Raghu Prasad Rao; Reddy, Boojala Vijay B. (BioMed Central, 2009-01-08)
    Cold adapted or psychrophilic organisms grow at low temperatures, where most of other organisms cannot grow. This adaptation requires a vast array of sequence, structural and physiological adjustments. To understand the ...