Using stable isotope probing to characterize differences between free-living and sediment-associated microorganisms in the subsurface
MetadataShow full item record
KeywordSubsurface microbiology; Stable-isotope probing; Top-down control; Microbial community structure
Aquifers are subterranean reservoirs of freshwater with heterotrophic bacterial communities attached to the sediments and free-living in the groundwater. In the present study, mesocosms were used to assess factors controlling the diversity and activity of the subsurface bacterial community. The assimilation of 13C, derived from 13C-acetate, was monitored to determine whether the sediment-associated and free-living bacterial community would respond similarly to the presence of protozoan grazers. We observed a dynamic response in the sediment-associated bacterial community and none in the free-living community. The disparity in these observations highlights the importance of the sediment-associated bacterial community in the subsurface carbon cycle.
Author Posting. © The Author(s), 2012. This is the author's version of the work. It is posted here by permission of Taylor & Francis for personal use, not for redistribution. The definitive version was published in Geomicrobiology Journal (2013): 362-370, doi:10.1080/01490451.2012.689090.
Suggested CitationPreprint: Longnecker, Krista, Kujawinski, Elizabeth B., "Using stable isotope probing to characterize differences between free-living and sediment-associated microorganisms in the subsurface", 2012-04-24, https://doi.org/10.1080/01490451.2012.689090, https://hdl.handle.net/1912/5882
Showing items related by title, author, creator and subject.
Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° North using 13C DNA Stable Isotope Probing and Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization Richberg, Kevin P. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2010-09)At deep‐sea hydrothermal vents chemolithoautotrophic microbes mediate the transfer of geothermal chemical energy to higher trophic levels. To better understand these underlying processes and the organisms catalyzing them, ...
Osmium isotope systematics of the Proterozoic and Phanerozoic ophiolitic chromitites : in situ ion probe analysis of primary Os-rich PGM Ahmed, Ahmed H.; Hanghoj, Karen; Kelemen, Peter B.; Hart, Stanley R.; Arai, Shoji (2006-03-17)In-situ 187Os/188Os ratios are determined on Os-rich platinum-group minerals in podiform chromitites both in the Proterozoic ophiolite, Eastern Desert, Egypt, and in the Phanerozoic Oman ophiolite. Because they have very ...
C and N stable isotopes and elemental ratios of high and low molecular weight (HMW, LMW) DOM collected from the North Pacific Subtropical Gyre and Central North Atlantic determined by elemental analyzer isotope ratio mass spectrometry (EA-IRMS) McCarthy, Matthew D.; Guilderson, Thomas (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: email@example.com, 2020-05-20)C and N stable isotopes and elemental ratios of high and low molecular weight (HMW, LMW) DOM collected from the North Pacific Subtropical Gyre and Central North Atlantic determined by elemental analyzer isotope ratio mass ...