Show simple item record

dc.contributor.authorKrasitskaya, Vasilisa V.  Concept link
dc.contributor.authorKudryavtsev, Alexander N.  Concept link
dc.contributor.authorShimomura, Osamu  Concept link
dc.contributor.authorFrank, Ludmila A.  Concept link
dc.date.accessioned2013-03-22T15:02:24Z
dc.date.available2014-10-22T08:57:23Z
dc.date.issued2012-11-29
dc.identifier.citationAnalytical Methods 5 (2013): 636-640en_US
dc.identifier.urihttps://hdl.handle.net/1912/5820
dc.descriptionAuthor Posting. © Royal Society of Chemistry, 2013. This article is posted here by permission of RSC Publishing for personal use, not for redistribution. The definitive version was published in Analytical Methods 5 (2013): 636-640, doi:10.1039/C2AY25976A.en_US
dc.description.abstractTwo obelin mutants distinctly different in the color of bioluminescence were successfully applied to simultaneous detection of two analytes in a single well. The flash-type signals were triggered by single injection of Ca2+ and were discriminated by the spectral and time resolutions. The technique was developed in a high-throughput format, and applied to simultaneous immunoassay of two gonadotropic hormones – luteinizing (lutropin or hLH) and follicle stimulating (hFSH) – in clinical sera, and to simultaneous detection of two gene allelic variants at single nucleotide polymorphism genotyping of the human F5 gene encoding factor V Leiden polymorphism 1691 G/A (R506Q). A considerable number of samples were investigated and the results obtained were in good correlation with those obtained by using traditional techniques.en_US
dc.description.sponsorshipThis work was supported by the Program of the Government of Russian Federation “Measures to attract leading scientists to Russian educational institutions” (grant no. 11. G34.31.058).en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherRSC Publishingen_US
dc.relation.urihttps://doi.org/10.1039/C2AY25976A
dc.titleObelin mutants as reporters in bioluminescent dual-analyte binding assayen_US
dc.typeArticleen_US
dc.description.embargo2013-11-29en_US
dc.identifier.doi10.1039/C2AY25976A


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record