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dc.contributor.authorBilogan, Cassandra K.  Concept link
dc.contributor.authorHorb, Marko E.  Concept link
dc.date.accessioned2013-02-27T18:25:25Z
dc.date.available2013-02-27T18:25:25Z
dc.date.issued2012-07
dc.identifier.urihttps://hdl.handle.net/1912/5790
dc.descriptionAuthor Posting. © The Author(s), 2012. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in Genesis 50 (2012): 853-870, doi:10.1002/dvg.22048.en_US
dc.description.abstractPancreas specific transcription factor 1a (Ptf1a), a bHLH transcription factor, has two temporally distinct functions during pancreas development; initially it is required for early specification of the entire pancreas, while later it is required for proper differentiation and maintenance of only acinar cells. The importance of Ptf1a function was revealed by the fact that loss of Ptf1a leads to pancreas agenesis in humans. While Ptf1a is one of the most important pancreatic transcription factors, little is known about the differences between the regulatory networks it controls during initial specification of the pancreas as opposed to acinar cell development, and to date no comprehensive analysis of its downstream targets has been published. In this paper, we use Xenopus embryos to identify putative downstream targets of Ptf1a. We isolated anterior endoderm tissue overexpressing Ptf1a at two early stages, NF32 and NF36, and compared their gene expression profiles using microarrays. Our results revealed that Ptf1a regulates genes with a wide variety of functions, providing insight into the complexity of the regulatory network required for pancreas specification.en_US
dc.description.sponsorshipNational Institutes of Health (DK077197)en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.relation.urihttps://doi.org/10.1002/dvg.22048
dc.subjectXenopusen_US
dc.subjectEndodermen_US
dc.subjectPtf1aen_US
dc.subjectMicroarrayen_US
dc.subjectPancreas developmenten_US
dc.titleMicroarray analysis of Xenopus endoderm expressing Ptf1aen_US
dc.typePreprinten_US


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