Probing the function of neuronal populations : combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging
Kee, Michelle Z. L.
Loew, Leslie M.
Augustine, George J.
MetadataShow full item record
KeywordOptogenetics; Channelrhodopsin; Digital micromirror device; Voltage-sensitive dye imaging; Inhibitory circuitry; Cerebellum
Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical detection of resulting postsynaptic activity. Such technology allowed us to control the activity of cerebellar interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, their postsynaptic targets. This approach should substantially accelerate our understanding of information processing by populations of neurons within brain circuits.
Author Posting. © The Author(s), 2012. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Neuroscience Research 75 (2013): 76-81, doi:10.1016/j.neures.2012.11.006.
Suggested CitationPreprint: Tsuda, Sachiko, Kee, Michelle Z. L., Cunha, Catarina, Kim, Jinsook, Yan, Ping, Loew, Leslie M., Augustine, George J., "Probing the function of neuronal populations : combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging", 2012-12-04, https://doi.org/10.1016/j.neures.2012.11.006, https://hdl.handle.net/1912/5683
Showing items related by title, author, creator and subject.
All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation Lo, Shun Qiang; Koh, Dawn X. P.; Sng, Judy C. G.; Augustine, George J. (SPIE, 2015-03-31)We describe an experimental approach that uses light to both control and detect neuronal activity in mouse barrel cortex slices: blue light patterned by a digital micromirror array system allowed us to photostimulate ...
Berglund, Ken; Wen, Lei; Dunbar, Robert L.; Feng, Guoping; Augustine, George J. (Society for Neuroscience, 2016-05-25)Tonic inhibition was imaged in cerebellar granule cells of transgenic mice expressing the optogenetic chloride indicator, Clomeleon. Blockade of GABAA receptors substantially reduced chloride concentration in granule cells ...
Asrican, Brent; Augustine, George J.; Berglund, Ken; Chen, Susu; Chow, Nick; Deisseroth, Karl; Feng, Guoping; Gloss, Bernd; Hira, Riichiro; Hoffmann, Carolin; Kasai, Haruo; Katarya, Malvika; Kim, Jinsook; Kudolo, John; Lee, Li Ming; Lo, Shun Qiang; Mancuso, James; Matsuzaki, Masanori; Nakajima, Ryuichi; Qiu, Li; Tan, Gregory; Tang, Yanxia; Ting, Jonathan T.; Tsuda, Sachiko; Wen, Lei; Zhang, Xuying; Zhao, Shengli (Frontiers Media, 2013-11-26)Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of ...