Mutant analysis of luminescence and autoinduction in a marine bacterium
Kuo, Alan J.
MetadataShow full item record
The marine symbiotic bacterium Vibrio fischeri is striking for its ability both to emit light and to dramatically regulate light emission using a cell-to-cell signalling mechanism called autoinduction. The latter is mediated by a signal molecule called the "autoinducer". The mechanistic bases of both luminescence and autoinduction are well known in V. fischeri, but this knowledge is mostly derived from studies of the cloned luminescence and autoinduction genes expressed in Escherichia coli. In this study, luminescence and autoinduction mutations were systematically generated in V. fischeri to explore aspects of luminescence and autoinduction not addressable in E. coli, such as the adaptive significance of luminescence. Most dramatically, the mutants revealed the presence of multiple autoinducers and autoinducer synthases in V. fischeri. One of the autoinducers (autoinducer-2, or AI-2) was chemically purified and shown to be Noctanoyi- L-homoserine lactone. The genetic locus encoding the AI-2 synthase was cloned and designated ain (autoinducer). Manipulation of ain and AI-2 in V. jischeri demonstrated that the function of AI-2 appears to be to inhibit rather than to promote autoinduction.
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution December 1994
Suggested CitationThesis: Kuo, Alan J., "Mutant analysis of luminescence and autoinduction in a marine bacterium", 1994-12, DOI:10.1575/1912/5626, https://hdl.handle.net/1912/5626
Showing items related by title, author, creator and subject.
A qualitative and quantitative study of the distribution of pelagic sediment in the Atlantic Basin Webb, Helen F. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1997-02)Pelagic sedimentation is the primary modifier of topography generated by ridge-associated volcanic and tectonic processes. This thesis represents an effort to understand the processes of, and the general distribution of, ...
Acoustic scattering by axisymmetric finite-length bodies with application to fish : measurement and modeling Reeder, D. Benjamin (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2002-06)This thesis investigates the complexities of acoustic scattering by finite bodies in general and by fish in particular through the development of an advanced acoustic scattering model and detailed laboratory acoustic ...
Nadakuditi, Rajesh Rao (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2002-02)One of the major problems in wireless communications is compensating for the time-varying intersymbol interference (ISI) due to multipath. Underwater acoustic communications is one such type of wireless communications ...