Nematostella Embryonic Transcriptome
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RNA-Seq was performed on Nematostella Embryos at 5 timepoints during early development: 0hrs, 6hrs, 12hrs, 18hrs, 24hrs after fertilization. Embryos were harvested, lysed and mRNAs were selected using Dynabeads. Directional sequencing libraries were constructed using the ScriptSeq kit from Epicentre. A control RNA set from the National Institute of Standards and Technology (NIST) were spiked-into the library preparation. Libraries were sequenced using the Illumina HiSeq 1000, version 3 chemistry, 100bp paired-end reads and produced over 200million reads. The reads were quality controlled filtered and assembled using the Trinity Assembler. This database contains the raw read files and the assembled Transcriptome.
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A quantitative reference transcriptome for Nematostella vectensis early embryonic development : a pipeline for de novo assembly in emerging model systems Tulin, Sarah; Aguiar, Derek; Istrail, Sorin; Smith, Joel (BioMed Central, 2013-06-03)The de novo assembly of transcriptomes from short shotgun sequences raises challenges due to random and non-random sequencing biases and inherent transcript complexity. We sought to define a pipeline for de novo transcriptome ...
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Biochemical changes associated with embryonic and larval development in the American lobster Homarus americanus Milne Edwards Sasaki, Glenn Craig (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1984-01)Proximate analysis was carried out on developing Homarus americanus Milne Edwards larvae and eggs. Lipids were further analyzed for class and fatty acid composition. During embryogenesis, triacylglycerol supplied most ...