Nematostella Embryonic Transcriptome
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2012-12-12Author
Tulin, Sarah
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Aguiar, Derek
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Istrail, Sorin
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Smith, Joel
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https://hdl.handle.net/1912/5613DOI
10.1575/1912/5613Abstract
RNA-Seq was performed on Nematostella Embryos at 5 timepoints during early development: 0hrs, 6hrs, 12hrs, 18hrs, 24hrs after fertilization. Embryos were harvested, lysed and mRNAs were selected using Dynabeads. Directional sequencing libraries were constructed using the ScriptSeq kit from Epicentre. A control RNA set from the National Institute of Standards and Technology (NIST) were spiked-into the library preparation. Libraries were sequenced using the Illumina HiSeq 1000, version 3 chemistry, 100bp paired-end reads and produced over 200million reads. The reads were quality controlled filtered and assembled using the Trinity Assembler. This database contains the raw read files and the assembled Transcriptome.
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Dataset: Tulin, Sarah, Aguiar, Derek, Istrail, Sorin, Smith, Joel, "Nematostella Embryonic Transcriptome", 2012-12-12, DOI:10.1575/1912/5613, https://hdl.handle.net/1912/5613Related items
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