Salt marsh sediment diversity : a test of the variability of the rare biosphere among environmental replicates


a service of the MBLWHOI Library | About WHOAS

Show simple item record

dc.contributor.author Bowen, Jennifer L.
dc.contributor.author Morrison, Hilary G.
dc.contributor.author Hobbie, John E.
dc.contributor.author Sogin, Mitchell L.
dc.date.accessioned 2012-12-07T16:26:41Z
dc.date.available 2012-12-28T09:42:25Z
dc.date.issued 2011-12-17
dc.identifier.uri http://hdl.handle.net/1912/5599
dc.description Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in The ISME Journal 6 (2012): 2014–2023, doi:10.1038/ismej.2012.47. en_US
dc.description.abstract Much of the phylogenetic diversity in microbial systems arises from rare taxa that comprise the long tail of taxon rank distribution curves. This vast diversity presents a challenge to testing hypotheses about the effects of perturbations on microbial community composition because within site variability of the rare taxa may be sufficiently large that it would require a prohibitive degree of sequencing to discern differences among samples. In this study we used pyrosequencing of 16S rRNA tags to examine the diversity and local scale variability of salt marsh sediment bacteria. Our goal was to determine whether pyrosequencing could produce similar patterns in community composition among replicate environmental samples from the same location. We hypothesized that repeated sampling from the same location would produce different snapshots of the rare community due to incomplete sequencing of the taxonomically rich rare biosphere. The concern was that variation resulting from incomplete sequencing could mask subtle community shifts caused by environmental perturbations. Our data indicate that salt marsh sediments contain a remarkably diverse array of bacterial taxa and, in contrast to our hypothesis, repeated sampling from within the same site produces reliably similar patterns in bacterial community composition, even among rare organisms. These results demonstrate that deep sequencing of 16s tags is well suited to distinguish site-specific similarities and differences among rare taxa and is a valuable tool for hypothesis testing in microbial ecology. en_US
dc.description.sponsorship Funding for this research came from the National Science Foundation (DEB- 0717155 to JEH and HGM, and award DBI-0400819 to JLB). en_US
dc.format.mimetype application/pdf
dc.language.iso en_US en_US
dc.relation.uri http://dx.doi.org/10.1038/ismej.2012.47
dc.subject Pyrosequencing en_US
dc.subject Salt marshes en_US
dc.subject Microbial diversity en_US
dc.subject Rare biosphere en_US
dc.title Salt marsh sediment diversity : a test of the variability of the rare biosphere among environmental replicates en_US
dc.type Preprint en_US
dc.description.embargo 2012-12-28 en_US

Files in this item

This item appears in the following Collection(s)

Show simple item record

Search WHOAS


My Account