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Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin

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dc.contributor.author Li, Wenjing
dc.contributor.author Moriwaki, Takashi
dc.contributor.author Tani, Tomomi
dc.contributor.author Watanabe, Takashi
dc.contributor.author Kaibuchi, Kozo
dc.contributor.author Goshima, Gohta
dc.date.accessioned 2012-12-06T18:50:32Z
dc.date.available 2013-05-26T08:50:59Z
dc.date.issued 2012-11-26
dc.identifier.citation Journal of Cell Biology 199 (2012): 849-862 en_US
dc.identifier.uri http://hdl.handle.net/1912/5596
dc.description © The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Cell Biology 199 (2012): 849-862, doi:10.1083/jcb.201206101. en_US
dc.description.abstract Dynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs. However, the molecular activity of each protein and the basis of the modulation of MT dynamics by these three factors are unknown. In this paper, we showed in vitro that XMAP215msps had a potent growth-promoting activity at a wide range of tubulin concentrations, whereas Sentin, when recruited by EB1 to the growing MT tip, accelerated growth and also increased catastrophe frequency. When all three factors were combined, the growth rate was synergistically enhanced, and rescue events were observed most frequently, but frequent catastrophes restrained the lengthening of the MTs. We propose that MT dynamics are promoted by the independent as well as the cooperative action of XMAP215msps polymerase and the EB1–Sentin duo. en_US
dc.description.sponsorship This work was supported by a Next Generation grant (Japan Society for the Promotion of Science), the Inoue Foundation, and the Human Frontier Science Program (to G. Goshima). W. Li was supported by the Global Centers of Excellence program, the Leading Graduate School program, and the State Scholarship Study Abroad Program of the Chinese Scholarship Council. en_US
dc.format.mimetype application/pdf
dc.language.iso en_US en_US
dc.publisher Rockefeller University Press en_US
dc.relation.uri http://dx.doi.org/10.1083/jcb.201206101
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ *
dc.title Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin en_US
dc.type Article en_US
dc.description.embargo 2013-05-26 en_US
dc.identifier.doi 10.1083/jcb.201206101


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