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dc.contributor.authorLi, Wenjing
dc.contributor.authorMoriwaki, Takashi
dc.contributor.authorTani, Tomomi
dc.contributor.authorWatanabe, Takashi
dc.contributor.authorKaibuchi, Kozo
dc.contributor.authorGoshima, Gohta
dc.date.accessioned2012-12-06T18:50:32Z
dc.date.available2014-10-22T08:57:23Z
dc.date.issued2012-11-26
dc.identifier.citationJournal of Cell Biology 199 (2012): 849-862en_US
dc.identifier.urihttp://hdl.handle.net/1912/5596
dc.description© The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Cell Biology 199 (2012): 849-862, doi:10.1083/jcb.201206101.en_US
dc.description.abstractDynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs. However, the molecular activity of each protein and the basis of the modulation of MT dynamics by these three factors are unknown. In this paper, we showed in vitro that XMAP215msps had a potent growth-promoting activity at a wide range of tubulin concentrations, whereas Sentin, when recruited by EB1 to the growing MT tip, accelerated growth and also increased catastrophe frequency. When all three factors were combined, the growth rate was synergistically enhanced, and rescue events were observed most frequently, but frequent catastrophes restrained the lengthening of the MTs. We propose that MT dynamics are promoted by the independent as well as the cooperative action of XMAP215msps polymerase and the EB1–Sentin duo.en_US
dc.description.sponsorshipThis work was supported by a Next Generation grant (Japan Society for the Promotion of Science), the Inoue Foundation, and the Human Frontier Science Program (to G. Goshima). W. Li was supported by the Global Centers of Excellence program, the Leading Graduate School program, and the State Scholarship Study Abroad Program of the Chinese Scholarship Council.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherRockefeller University Pressen_US
dc.relation.urihttp://dx.doi.org/10.1083/jcb.201206101
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/*
dc.titleReconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentinen_US
dc.typeArticleen_US
dc.description.embargo2013-05-26en_US
dc.identifier.doi10.1083/jcb.201206101


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