Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin

View/ Open
Date
2012-11-26Author
Li, Wenjing
Concept link
Moriwaki, Takashi
Concept link
Tani, Tomomi
Concept link
Watanabe, Takashi
Concept link
Kaibuchi, Kozo
Concept link
Goshima, Gohta
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/5596As published
https://doi.org/10.1083/jcb.201206101DOI
10.1083/jcb.201206101Abstract
Dynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs. However, the molecular activity of each protein and the basis of the modulation of MT dynamics by these three factors are unknown. In this paper, we showed in vitro that XMAP215msps had a potent growth-promoting activity at a wide range of tubulin concentrations, whereas Sentin, when recruited by EB1 to the growing MT tip, accelerated growth and also increased catastrophe frequency. When all three factors were combined, the growth rate was synergistically enhanced, and rescue events were observed most frequently, but frequent catastrophes restrained the lengthening of the MTs. We propose that MT dynamics are promoted by the independent as well as the cooperative action of XMAP215msps polymerase and the EB1–Sentin duo.
Description
© The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Cell Biology 199 (2012): 849-862, doi:10.1083/jcb.201206101.
Collections
Suggested Citation
Journal of Cell Biology 199 (2012): 849-862The following license files are associated with this item:
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 3.0 Unported
Related items
Showing items related by title, author, creator and subject.
-
Escherichia coli K-12 : a cooperatively developed annotation snapshot—2005
Riley, Monica; Abe, Takashi; Arnaud, Martha B.; Berlyn, Mary K. B.; Blattner, Frederick R.; Chaudhuri, Roy R.; Glasner, Jeremy D.; Horiuchi, Takashi; Keseler, Ingrid M.; Kosuge, Takehide; Mori, Hirotada; Perna, Nicole T.; Plunkett, Guy; Rudd, Kenneth E.; Serres, Margrethe H.; Thomas, Gavin H.; Thomson, Nicholas R.; Wishart, David; Wanner, Barry L. (Oxford University Press, 2006-01-05)The goal of this group project has been to coordinate and bring up-to-date information on all genes of Escherichia coli K-12. Annotation of the genome of an organism entails identification of genes, the boundaries of genes ... -
Five factors can reconstitute all three phases of microtubule polymerization dynamics
Moriwaki, Takashi; Goshima, Gohta (Rockefeller University Press, 2016-10-31)Cytoplasmic microtubules (MTs) undergo growth, shrinkage, and pausing. However, how MT polymerization cycles are produced and spatiotemporally regulated at a molecular level is unclear, as the entire cycle has not been ... -
Water properties, heat and volume fluxes of Pacific water in Barrow Canyon during summer 2010
Itoh, Motoyo; Pickart, Robert S.; Kikuchi, Takashi; Fukamachi, Yasushi; Ohshima, Kay I.; Simizu, Daisuke; Arrigo, Kevin R.; Vagle, Svein; He, Jianfeng; Ashjian, Carin J.; Mathis, Jeremy T.; Nishino, Shigeto; Nobre, Carolina (Elsevier, 2015-04-25)Over the past few decades, sea ice retreat during summer has been enhanced in the Pacific sector of the Arctic basin, likely due in part to increasing summertime heat flux of Pacific-origin water from the Bering Strait. ...