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dc.contributor.authorKapoor, Tarun M.  Concept link
dc.contributor.authorLampson, Michael A.  Concept link
dc.contributor.authorHergert, Polla  Concept link
dc.contributor.authorCameron, Lisa  Concept link
dc.contributor.authorCimini, Daniela  Concept link
dc.contributor.authorSalmon, Edward D.  Concept link
dc.contributor.authorMcEwen, Bruce F.  Concept link
dc.contributor.authorKhodjakov, Alexey  Concept link
dc.date.accessioned2006-02-07T18:39:38Z
dc.date.available2006-02-07T18:39:38Z
dc.date.issued2005-12-09
dc.identifier.urihttps://hdl.handle.net/1912/550
dc.descriptionAuthor Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of American Association for the Advancement of Science for personal use, not for redistribution. The definitive version was published in Science 311 (2006): 388-391, doi:10.1126/science.1122142.
dc.description.abstractThe stable propagation of genetic material during cell division depends on the congression of chromosomes to the spindle equator before the cell initiates anaphase. It is generally assumed that congression requires that chromosomes are connected to the opposite poles of the bipolar spindle (i.e., “bi-oriented”). We found that chromosomes can congress before becoming bioriented. By combining the use of reversible chemical inhibitors, live-cell light microscopy and correlative electron microscopy, we found that mono-oriented chromosomes could glide towards the spindle equator alongside kinetochore fibers attached to other already bi-oriented chromosomes. This congression mechanism depended on the kinetochore-associated plus enddirected microtubule motor CENP-E (kinesin-7).en
dc.description.sponsorshipSupported by grants from the NIH (GM59363 to A.K., GM65933 to T.M.K., GM24364 to E.D.S, and GM06627 to B.F.M)en
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dc.language.isoen_USen
dc.relation.urihttps://doi.org/10.1126/science.1122142
dc.titleChromosomes can congress to the metaphase plate before biorientationen
dc.typePreprinten


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