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dc.contributor.authorWhalen, Kristen E.
dc.contributor.authorReitzel, Adam M.
dc.contributor.authorHamdoun, Amro
dc.date.accessioned2012-10-01T17:59:11Z
dc.date.available2012-10-01T17:59:11Z
dc.date.issued2012-08-01
dc.identifier.citationMolecular Biology of the Cell 23 (2012): 3663-3672en_US
dc.identifier.urihttp://hdl.handle.net/1912/5408
dc.description© The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 23 (2012): 3663-3672, doi:10.1091/mbc.E12-06-0438.en_US
dc.description.abstractFertilization changes the structure and function of the cell surface. In sea urchins, these changes include polymerization of cortical actin and a coincident, switch-like increase in the activity of the multidrug efflux transporter ABCB1a. However, it is not clear how cortical reorganization leads to changes in membrane transport physiology. In this study, we used three-dimensional superresolution fluorescence microscopy to resolve the fine-scale movements of the transporter along polymerizing actin filaments, and we show that efflux activity is established after ABCB1a translocates to the tips of the microvilli. Inhibition of actin poly­merization or bundle formation prevents tip localization, resulting in the patching of ABCB1a at the cell surface and decreased efflux activity. In contrast, enhanced actin polymerization promotes tip localization. Finally, interference with Rab11, a regulator of apical recycling, inhibits activation of efflux activity in embryos. Together our results show that actin-mediated, short-range traffic and positioning of transporters at the cell surface regulates multidrug efflux activity and highlight the multifaceted roles of microvilli in the spatial distribution of membrane proteins.en_US
dc.description.sponsorshipFunding was provided by the National Institute of Child Health and Human Development (058070), the National Center for Research Resources (P30-NS047101), the Krinsk Research Advancement Initiative to A.H., a Scripps Postdoctoral Scholar Fellowship to K.W., and NICHD award 062178 to A.M.R.en_US
dc.format.mimetypeapplication/pdf
dc.format.mimetypevideo/quicktime
dc.language.isoen_USen_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.relation.urihttp://dx.doi.org/10.1091/mbc.E12-06-0438
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0*
dc.titleActin polymerization controls the activation of multidrug efflux at fertilization by translocation and fine-scale positioning of ABCB1 on microvillien_US
dc.typeArticleen_US
dc.identifier.doi10.1091/mbc.E12-06-0438


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