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dc.contributor.authorRieder, Conly L.  Concept link
dc.date.accessioned2006-02-01T21:21:28Z
dc.date.available2006-02-01T21:21:28Z
dc.date.issued2005-09-12
dc.identifier.urihttps://hdl.handle.net/1912/538
dc.descriptionAuthor Posting. © The Author, 2005. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Chromosoma 114 (2005): 310-318, doi:10.1007/s00412-005-0028-2.
dc.description.abstractThe attachment to and movement of a chromosome on the mitotic spindle is mediated by the formation of a bundle of microtubules (MTs) that tethers the kinetochore on the chromosome to a spindle pole. The origin of these “kinetochore fibers” (K-fibers) has been investigated for over 125 years. As noted in 1944 by Schrader, there are only three possible ways to form a K-fiber: either it a) grows from the pole until it contacts the kinetochore; b) grows directly from the kinetochore; or c) it forms as a result of an interaction between the pole and the chromosome. Since Schrader’s time it has been firmly established that K-fibers in centrosome-containing animal somatic cells form as kinetochores capture MTs growing from the spindle pole (route a). It is now similarly clear that in cells lacking centrosomes, including plants and many animal oocytes, K-fibers “self-assemble” from MTs generated by the chromosomes (route b). Can animal somatic cells form K-fibers in the absence of centrosomes by the “self-assembly” pathway? In 2000 the answer to this question was shown to be a resounding “yes”. With this result, the next question became whether the presence of a centrosome normally suppresses K-fiber self-assembly, or if this route works concurrently with centrosome-mediated K-fiber formation. This question, too, has recently been answered: observations on untreated live animal cells expressing GFP-tagged tubulin clearly show that kinetochores can nucleate the formation of their associated MTs in the presence of functional centrosomes. The concurrent operation of these two “dueling” routes for forming K-fibers in animals helps explain why the attachment of kinetochores and the maturation of K-fibers occur as quickly as it does on all chromosomes within a cell.en
dc.description.sponsorshipThe work is sponsored by NIH grant GMS 40198.en
dc.format.extent463425 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.relation.urihttps://doi.org/10.1007/s00412-005-0028-2
dc.titleKinetochore fiber formation in animal somatic cells : dueling mechanisms come to a drawen
dc.typePreprinten


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