Pac-man motility of kinetochores unleashed by laser microsurgery


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dc.contributor.author LaFountain, James R.
dc.contributor.author Cohan, Christopher S.
dc.contributor.author Oldenbourg, Rudolf
dc.date.accessioned 2012-08-28T14:08:50Z
dc.date.available 2012-08-28T14:08:50Z
dc.date.issued 2012-06-27
dc.identifier.citation Molecular Biology of the Cell 23 (2012): 3133-3142 en_US
dc.identifier.uri http://hdl.handle.net/1912/5344
dc.description © The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molecular Biology of the Cell 23 (2012): 3133-3142, doi:10.1091/mbc.E12-04-0314. en_US
dc.description.abstract We report on experiments directly in living cells that reveal the regulation of kinetochore function by tension. X and Y sex chromosomes in crane fly (Nephrotoma suturalis) spermatocytes exhibit an atypical segregation mechanism in which each univalent maintains K-fibers to both poles. During anaphase, each maintains a leading fiber (which shortens) to one pole and a trailing fiber (which elongates) to the other. We used this intriguing behavior to study the motile states that X-Y kinetochores are able to support during anaphase. We used a laser microbeam to either sever a univalent along the plane of sister chromatid cohesion or knock out one of a univalent's two kinetochores to release one or both from the resistive influence of its sister's K-fiber. Released kinetochores with attached chromosome arms moved poleward at rates at least two times faster than normal. Furthermore, fluorescent speckle microscopy revealed that detached kinetochores converted their functional state from reverse pac-man to pac-man motility as a consequence of their release from mechanical tension. We conclude that kinetochores can exhibit pac-man motility, even though their normal behavior is dominated by traction fiber mechanics. Unleashing of kinetochore motility through loss of resistive force is further evidence for the emerging model that kinetochores are subject to tension-sensitive regulation. en_US
dc.description.sponsorship This work was supported by a grant to R.O. from the National Institute of Biomedical Imaging and Bioengineering (R01EB002045). en_US
dc.format.mimetype application/pdf
dc.format.mimetype video/quicktime
dc.language.iso en_US en_US
dc.publisher American Society for Cell Biology en_US
dc.relation.uri http://dx.doi.org/10.1091/mbc.E12-04-0314
dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/us/ *
dc.title Pac-man motility of kinetochores unleashed by laser microsurgery en_US
dc.type Article en_US
dc.identifier.doi 10.1091/mbc.E12-04-0314

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