Now showing items 1-10 of 57
Reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction
Crawling movement in eukaryotic cells requires coordination of leading edge protrusion with cell body retraction [1-3]. Protrusion is driven by actin polymerization along the leading edge . The mechanism of retraction ...
Mapping optical path length and image enhancement using quantitative orientation-independent differential interference contrast microscopy
We describe the principles of using orientation-independent differential interference contrast (OI-DIC) microscopy for mapping optical path length (OPL). Computation of the scalar two-dimensional OPL map is based on an ...
Superresolution by image scanning microscopy using pixel reassignment
(Optical Society of America, 2013-07-30)
The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes ...
Self-organization of actin filament orientation in the dendritic-nucleation/array-treadmilling model
The dendritic-nucleation/array-treadmilling model provides a conceptual framework for the generation of the actin network driving motile cells. We have incorporated it into a 2-D, stochastic computer model to study ...
A fiber-optic phase-randomizer for microscope illumination by laser
(Rockefeller University Press, 1979-11)
Microtubule dynamics in cell division : exploring living cells with polarized light microscopy
This Perspective is an account of my early experience while I studied the dynamic organization and behavior of the mitotic spindle and its submicroscopic filaments using polarized light microscopy. The birefringence of ...
Multiplexed spectral imaging of 120 different fluorescent labels
(Public Library of Science, 2016-07-08)
The number of fluorescent labels that can unambiguously be distinguished in a single image when acquired through band pass filters is severely limited by the spectral overlap of available fluorophores. The recent development ...
Mammalian end binding proteins control persistent microtubule growth
(Rockefeller University Press, 2009-03-02)
End binding proteins (EBs) are highly conserved core components of microtubule plus-end tracking protein networks. Here we investigated the roles of the three mammalian EBs in controlling microtubule dynamics and analyzed ...
Mapping the biosphere : exploring species to understand the origin, organization and sustainability of biodiversity
(Taylor & Francis, 2012-03-27)
The time is ripe for a comprehensive mission to explore and document Earth's species. This calls for a campaign to educate and inspire the next generation of professional and citizen species explorers, investments in ...
Pac-man motility of kinetochores unleashed by laser microsurgery
(American Society for Cell Biology, 2012-06-27)
We report on experiments directly in living cells that reveal the regulation of kinetochore function by tension. X and Y sex chromosomes in crane fly (Nephrotoma suturalis) spermatocytes exhibit an atypical segregation ...