Now showing items 1-7 of 7

  • Bipolarization and poleward flux correlate during xenopus extract spindle assembly 

    Mitchison, Timothy J.; Maddox, P.; Groen, Aaron C.; Cameron, L.; Perlman, Z.; Ohi, Ryoma; Desai, A.; Salmon, Edward D.; Kapoor, Tarun M. (American Society for Cell Biology, 2004-09-22)
    We investigated the mechanism by which meiotic spindles become bipolar and the correlation between bipolarity and poleward flux, using Xenopus egg extracts. By speckle microscopy and computational alignment, we find that ...
  • Chromosomes can congress to the metaphase plate before biorientation 

    Kapoor, Tarun M.; Lampson, Michael A.; Hergert, Polla; Cameron, Lisa; Cimini, Daniela; Salmon, Edward D.; McEwen, Bruce F.; Khodjakov, Alexey (2005-12-09)
    The stable propagation of genetic material during cell division depends on the congression of chromosomes to the spindle equator before the cell initiates anaphase. It is generally assumed that congression requires that ...
  • Directly probing the mechanical properties of the spindle and its matrix 

    Gatlin, Jesse C.; Matov, Alexandre; Danuser, Gaudenz; Mitchison, Timothy J.; Salmon, Edward D. (Rockefeller University Press, 2010-02-22)
    Several recent models for spindle length regulation propose an elastic pole to pole spindle matrix that is sufficiently strong to bear or antagonize forces generated by microtubules and microtubule motors. We tested this ...
  • Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly 

    Groen, Aaron C.; Maresca, Thomas J.; Gatlin, Jesse C.; Salmon, Edward D.; Mitchison, Timothy J. (American Society for Cell Biology, 2009-04-15)
    Distinct pathways from centrosomes and chromatin are thought to contribute in parallel to microtubule nucleation and stabilization during animal cell mitotic spindle assembly, but their full mechanisms are not known. We ...
  • Microtubule plus-end dynamics in Xenopus egg extract spindles 

    Tirnauer, Jennifer S.; Salmon, Edward D.; Mitchison, Timothy J. (American Society for Cell Biology, 2004-02-06)
    Microtubule dynamics underlie spindle assembly, yet we do not know how the spindle environment affects these dynamics. We developed methods for measuring two key parameters of microtubule plus-end dynamic instability in ...
  • Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles 

    Mitchison, Timothy J.; Maddox, P.; Gaetz, J.; Groen, Aaron C.; Shirasu, M.; Desai, A.; Salmon, Edward D.; Kapoor, Tarun M. (American Society for Cell Biology, 2005-03-23)
    Metaphase spindles assemble to a steady state in length by mechanisms that involve microtubule dynamics and motor proteins, but they are incompletely understood. We found that Xenopus extract spindles recapitulate the ...
  • Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity 

    Maresca, Thomas J.; Groen, Aaron C.; Gatlin, Jesse C.; Ohi, Ryoma; Mitchison, Timothy J.; Salmon, Edward D. (2009-05)
    During animal cell division, a gradient of GTP-bound Ran is generated around mitotic chromatin. It is generally accepted that this RanGTP gradient is essential for organizing the spindle since it locally activates critical ...