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Browsing Cellular Dynamics Program by Title

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Browsing Cellular Dynamics Program by Title

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  • Mitchison, Timothy J.; Maddox, P.; Groen, Aaron C.; Cameron, L.; Perlman, Z.; Ohi, Ryoma; Desai, A.; Salmon, Edward D.; Kapoor, Tarun M. (American Society for Cell Biology, 2004-09-22)
    We investigated the mechanism by which meiotic spindles become bipolar and the correlation between bipolarity and poleward flux, using Xenopus egg extracts. By speckle microscopy and computational alignment, we find that ...
  • Goda, Makoto; Inoue, Shinya; Mabuchi, Issei (Marine Biological Laboratory, 2009-02)
    Calyculin-A (CLA), a protein phosphatase inhibitor, has been known to induce cleavage resembling normal furrowing in unfertilized sea urchin eggs. In CLA-treated eggs, actin filaments and myosin assemble to form a contractile ...
  • Janicke, Marie A.; Lasko, Loren; Oldenbourg, Rudolf; LaFountain, James R. (American Society for Cell Biology, 2007-02-21)
    This study investigated the basis of meiosis II nondisjunction. Cold arrest induced a fraction of meiosis II crane fly spermatocytes to form (n + 1) and (n – 1) daughters during recovery. Live-cell liquid crystal polarized ...
  • Kapoor, Tarun M.; Lampson, Michael A.; Hergert, Polla; Cameron, Lisa; Cimini, Daniela; Salmon, Edward D.; McEwen, Bruce F.; Khodjakov, Alexey (2005-12-09)
    The stable propagation of genetic material during cell division depends on the congression of chromosomes to the spindle equator before the cell initiates anaphase. It is generally assumed that congression requires that ...
  • Dumont, Sophie; Mitchison, Timothy J. (2009-05)
    Although the molecules involved in mitosis are becoming better characterized, we still lack an understanding of the emergent mechanical properties of the mitotic spindle. For example, we cannot explain how spindle length ...
  • Wang, Yanfang; Zhu, Sha; Weisman, Gary A.; Gitlin, Jonathan D.; Petris, Michael J. (Public Library of Science, 2012-08-10)
    The transition metal, copper (Cu), is an enzymatic cofactor required for a wide range of biochemical processes. Its essentiality is demonstrated by Menkes disease, an X-linked copper deficiency disorder characterized by ...
  • Gatlin, Jesse C.; Matov, Alexandre; Danuser, Gaudenz; Mitchison, Timothy J.; Salmon, Edward D. (Rockefeller University Press, 2010-02-22)
    Several recent models for spindle length regulation propose an elastic pole to pole spindle matrix that is sufficiently strong to bear or antagonize forces generated by microtubules and microtubule motors. We tested this ...
  • Applewhite, Derek A.; Barzik, Melanie; Kojima, Shin-ichiro; Svitkina, Tatyana M.; Gertler, Frank B.; Borisy, Gary G. (American Society for Cell Biology, 2007-05-02)
    Filopodia have been implicated in a number of diverse cellular processes including growth-cone path finding, wound healing, and metastasis. The Ena/VASP family of proteins has emerged as key to filopodia formation but the ...
  • Barry, Edward; Hensel, Zach; Dogic, Zvonimir; Shribak, Michael; Oldenbourg, Rudolf (American Physical Society, 2006-01-11)
    We study the liquid-crystalline phase behavior of a concentrated suspension of helical flagella isolated from Salmonella typhimurium. Flagella are prepared with different polymorphic states, some of which have a pronounced ...
  • Nozaki, Tadasu; Kaizu, Kazunari; Pack, Chan-Gi; Tamura, Sachiko; Tani, Tomomi; Hihara, Saera; Nagai, Takeharu; Takahashi, Koichi; Maeshima, Kazuhiro (Landes Bioscience, 2013-08-12)
    Genomic DNA is organized three dimensionally within cells as chromatin and is searched and read by various proteins by an unknown mechanism; this mediates diverse cell functions. Recently, several pieces of evidence, ...
  • Dumont, Sophie; Mitchison, Timothy J. (2009-07)
    The mitotic spindle assembles to a steady-state length at metaphase through the integrated action of molecular mechanisms that generate and respond to mechanical forces. While molecular mechanisms that produce force have ...
  • Groen, Aaron C.; Maresca, Thomas J.; Gatlin, Jesse C.; Salmon, Edward D.; Mitchison, Timothy J. (American Society for Cell Biology, 2009-04-15)
    Distinct pathways from centrosomes and chromatin are thought to contribute in parallel to microtubule nucleation and stabilization during animal cell mitotic spindle assembly, but their full mechanisms are not known. We ...
  • LaFountain, James R.; Cohan, Christopher S.; Oldenbourg, Rudolf (American Society of Cell Biology, 2011-10-26)
    The impact of mechanical forces on kinetochore motility was investigated using laser microsurgery to detach kinetochores with associated chromatin (K fragment) from meiotic chromosomes in spermatocytes from the crane fly ...
  • Aratyn, Yvonne S.; Schaus, Thomas E.; Taylor, Edwin W.; Borisy, Gary G. (American Society for Cell Biology, 2007-08-01)
    Recent studies showed that the actin cross-linking protein, fascin, undergoes rapid cycling between filopodial filaments. Here, we used an experimental and computational approach to dissect features of fascin exchange and ...
  • Pulido, Camila; Malagon, Gerardo; Ferrer, Camilo; Chen, Jun Kui; Angueyra, Juan Manuel; Nasi, Enrico; Gomez, Maria del Pilar (Rockefeller University Press, 2011-12-26)
    Two types of microvillar photoreceptors in the neural tube of amphioxus, an early chordate, sense light via melanopsin, the same photopigment as in “circadian” light detectors of higher vertebrates. Because in amphioxus ...
  • LaFountain, James R.; Oldenbourg, Rudolf (American Society for Cell Biology, 2004-09-22)
    To test the "traction fiber" model for metaphase positioning of bivalents during meiosis, kinetochore fibers of maloriented bivalents, induced during recovery from cold arrest, were analyzed with a liquid crystal polarizing ...
  • Komarova, Yulia A.; De Groot, Christian O.; Grigoriev, Ilya; Gouveia, Susana Montenegro; Munteanu, E. Laura; Schober, Joseph M.; Honnappa, Srinivas; Buey, Ruben M.; Hoogenraad, Casper C.; Dogterom, Marileen; Borisy, Gary G.; Steinmetz, Michel O.; Akhmanova, Anna (Rockefeller University Press, 2009-03-02)
    End binding proteins (EBs) are highly conserved core components of microtubule plus-end tracking protein networks. Here we investigated the roles of the three mammalian EBs in controlling microtubule dynamics and analyzed ...
  • Wheeler, Q. D.; Knapp, Sandra; Stevenson, D. W.; Stevenson, J.; Blum, Stan D.; Boom, B.. M.; Borisy, Gary G.; Buizer, J. L.; De Carvalho, M. R.; Cibrian, A.; Donoghue, M. J.; Doyle, V.; Gerson, E. M.; Graham, C. H.; Graves, P.; Graves, Sara J.; Guralnick, Robert P.; Hamilton, A. L.; Hanken, J.; Law, W.; Lipscomb, D. L.; Lovejoy, T. E.; Miller, Holly; Miller, J. S.; Naeem, S.; Novacek, M. J.; Page, L. M.; Platnick, N. I.; Porter-Morgan, H.; Raven, P. H.; Solis, M. A.; Valdecasas, A. G.; Van Der Leeuw, S.; Vasco, A.; Vermeulen, N.; Vogel, J.; Walls, R. L.; Wilson, E. O.; Woolley, J. B. (Taylor & Francis, 2012-03-27)
    The time is ripe for a comprehensive mission to explore and document Earth's species. This calls for a campaign to educate and inspire the next generation of professional and citizen species explorers, investments in ...
  • Pethig, Ronald; Schmueser, Ilka (Oslo Bioimpedance Group, 2012-11-23)
    Between 1910 and 1913 Rudolf Höber presented proof that the interiors of red blood cells and muscle cells contain conducting electrolytes, and that each conducting core is contained within an insulating membrane. He did ...
  • Inoue, Shinya (2008-04-11)
    This Perspective is an account of my early experience while I studied the dynamic organization and behavior of the mitotic spindle and its submicroscopic filaments using polarized light microscopy. The birefringence of ...

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