The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response


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dc.contributor.author Dyhrman, Sonya T.
dc.contributor.author Jenkins, Bethany D.
dc.contributor.author Rynearson, Tatiana A.
dc.contributor.author Saito, Mak A.
dc.contributor.author Mercier, Melissa L.
dc.contributor.author Alexander, Harriet
dc.contributor.author Whitney, LeAnn P.
dc.contributor.author Drzewianowski, Andrea
dc.contributor.author Bulygin, Vladimir V.
dc.contributor.author Bertrand, Erin M.
dc.contributor.author Wu, Zhijin
dc.contributor.author Benitez-Nelson, Claudia R.
dc.contributor.author Heithoff, Abigail
dc.date.accessioned 2012-06-26T18:16:29Z
dc.date.available 2012-06-26T18:16:29Z
dc.date.issued 2012-03-29
dc.identifier.citation PLoS One 7 (2012): e33768 en_US
dc.identifier.uri http://hdl.handle.net/1912/5242
dc.description © The Author(s), 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 7 (2012): e33768, doi:10.1371/journal.pone.0033768. en_US
dc.description.abstract Phosphorus (P) is a critical driver of phytoplankton growth and ecosystem function in the ocean. Diatoms are an abundant class of marine phytoplankton that are responsible for significant amounts of primary production. With the control they exert on the oceanic carbon cycle, there have been a number of studies focused on how diatoms respond to limiting macro and micronutrients such as iron and nitrogen. However, diatom physiological responses to P deficiency are poorly understood. Here, we couple deep sequencing of transcript tags and quantitative proteomics to analyze the diatom Thalassiosira pseudonana grown under P-replete and P-deficient conditions. A total of 318 transcripts were differentially regulated with a false discovery rate of <0.05, and a total of 136 proteins were differentially abundant (p<0.05). Significant changes in the abundance of transcripts and proteins were observed and coordinated for multiple biochemical pathways, including glycolysis and translation. Patterns in transcript and protein abundance were also linked to physiological changes in cellular P distributions, and enzyme activities. These data demonstrate that diatom P deficiency results in changes in cellular P allocation through polyphosphate production, increased P transport, a switch to utilization of dissolved organic P through increased production of metalloenzymes, and a remodeling of the cell surface through production of sulfolipids. Together, these findings reveal that T. pseudonana has evolved a sophisticated response to P deficiency involving multiple biochemical strategies that are likely critical to its ability to respond to variations in environmental P availability. en_US
dc.description.sponsorship This research was supported by the National Science Foundation (NSF) Environmental Genomics and NSF Biological Oceanography Program through grant OCE-0723667 to Dr. Dyhrman, Dr. Jenkins, Dr. Saito, and Dr. Rynearson, the NSF Chemical Oceanography Program through grant OCE-0549794 to Dr. Dyhrman and OCE-0526800 to Dr. Jenkins, the G. B. Moore Foundation and OCE-0752291 to Dr. Saito, NSF-EPSCoR (NSF-0554548 & NSF-1004057) to the University of Rhode Island, the Center for Microbial Oceanography: Research and Education, and the Joint Genome Institute/DOE Community Sequencing Program (CSP795793) to Dr. Jenkins, Dr. Dyhrman, Dr. Rynearson and Dr. Saito. en_US
dc.format.mimetype application/pdf
dc.language.iso en_US en_US
dc.publisher Public Library of Science en_US
dc.relation.uri http://dx.doi.org/10.1371/journal.pone.0033768
dc.rights.uri http://creativecommons.org/licenses/by/3.0/ *
dc.title The transcriptome and proteome of the diatom Thalassiosira pseudonana reveal a diverse phosphorus stress response en_US
dc.type Article en_US
dc.identifier.doi 10.1371/journal.pone.0033768

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