Insights into vitamin B12 production, acquisition, and use by marine microbes
Bertrand, Erin M.
MetadataShow full item record
The distribution and magnitude of marine primary production helps determine the ocean's role in global carbon cycling. Constraining factors that impact this productivity and elucidating selective pressures that drive the composition of marine microbial communities are thus essential aspects of marine biogeochemistry. Vitamin B12, also known as cobalamin, is a cobalt containing organometallic micronutrient produced by some bacteria and archaea and required by many eukaryotic phytoplankton for methionine biosynthesis and regeneration. Although the potential for vitamin B12 availability to impact primary production and phytoplankton species composition has long been recognized, the lack of molecular-level tools for studying B12 production, use and acquisition has limited inquiry into the role of the vitamin in marine biogeochemical processes. This thesis describes the development of such tools and implements them for the study of B12 dynamics in an Antarctic shelf ecosystem. Nucleic acid probes for B12 biosynthesis genes were designed and used to identify a potentially dominant group of B12 producers in the Ross Sea. The activity of this group was then verified by mass spectrometry-based peptide measurements. Then, possible interconnections between iron and B12 dynamics in this region were identified using field-based bottle incubation experiments and vitamin uptake measurements, showing that iron availability may impact both B12 production and consumption. Changes in diatom proteomes induced by low B12 and low iron availability were then examined and used to identify a novel B12 acquisition protein, CBA 1, in diatoms. This represents the first identification of a B12 acquisition protein in eukaryotic phytoplankton. Transcripts encoding CBAl were detected in natural phytoplankton communities, confirming that B12 acquisition is an important part of phytoplankton molecular physiology. Selected reaction monitoring mass spectrometry was used to measure the abundance of CBA 1 and methionine synthase proteins in diatoms cultures, revealing distinct protein abundance patterns as a function ofB12 availability. These peptide measurements were implemented to quantify methionine synthase proteins in McMurdo Sound, revealing that there is both B12 utilization and starvation in natural diatom communities and that these peptide measurements hold promise for revealing the metabolic status of marine ecosystems with respect to vitamin B12.
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2012
Suggested CitationThesis: Bertrand, Erin M., "Insights into vitamin B12 production, acquisition, and use by marine microbes", 2012-02, DOI:10.1575/1912/5200, https://hdl.handle.net/1912/5200
Showing items related by title, author, creator and subject.
Munson, Kathleen M. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2014-02)Methylation of mercury (Hg) in the marine water column has been hypothesized to serve as the primary source of the bioaccumulating chemical species monomethylmercury (MMHg) to marine food webs. Despite decades of research ...
Geiger, Sam R. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2000-09)A finite difference computer model is developed to simulate the exposure statistics of a radio frequency buoyant antenna as it is towed in a three-dimensional random seaway. The model allows the user to prescribe antenna ...
Approaches for assessing the presence and impact of thyroid hormone disrupting chemicals in delphinid cetaceans Montie, Eric W. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2006-09)Cetacean blubber is a primary site for lipid storage, which the animal utilizes during periods of energetic stress. It is important to understand how the blubber responds to factors such as ontogeny, water temperature, ...