Massive multiplication of genome and ribosomes in dormant cells (akinetes) of Aphanizomenon ovalisporum (Cyanobacteria)
Kaplan-Levy, Ruth N.
Mark Welch, Jessica L.
Post, Anton F.
MetadataShow full item record
KeywordAkinetes; Cyanobacteria; Dormancy; Fluorescence in situ hybridization; Polyphosphate; Polyploidy; Laser microdissection microscopy
Akinetes are dormancy cells commonly found among filamentous cyanobacteria, many of which are toxic and/or nuisance, bloom-forming species. Development of akinetes from vegetative cells is a process that involves morphological and biochemical modifications. Here we applied a single cell approach to quantify genome and ribosome content of akinetes and vegetative cells in Aphanizomenon ovalisporum (Cyanobacteria). Vegetative cells of A. ovalisporum were naturally polyploid and contained on average 8 genome copies per cell. However, the chromosomal content of akinetes increased up to 450 copies, with an average value of 119 genome copies per akinete, 15 fold higher that in vegetative cells. Based on fluorescence in situ hybridization with a probe targeting 16S rRNA and detection with confocal laser scanning microscopy we conclude that ribosomes accumulated in akinetes to a higher level than that found in vegetative cells. We further present evidence that this massive accumulation of nucleic acids in akinetes is likely supported by phosphate supplied from inorganic polyphosphate bodies that were abundantly present in vegetative cells, but notably absent from akinetes. These results are interpreted in the context of cellular investments for proliferation following long term dormancy, as the high nucleic acid content would provide the basis for extended survival, rapid resumption of metabolic activity and cell division upon germination.
Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in The ISME Journal 6 (2012): 670–679, doi:10.1038/ismej.2011.128.
Suggested CitationPreprint: Sukenik, Assaf, Kaplan-Levy, Ruth N., Mark Welch, Jessica L., Post, Anton F., "Massive multiplication of genome and ribosomes in dormant cells (akinetes) of Aphanizomenon ovalisporum (Cyanobacteria)", 2011-08, https://doi.org/10.1038/ismej.2011.128, https://hdl.handle.net/1912/5113
Showing items related by title, author, creator and subject.
Saito, Mak A. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2001-02)Processes that enable marine phytoplankton to acquire trace metals are fundamental to our understanding of primary productivity and global carbon cycling. This thesis explored the biogeochemistry of cobalt using analytical ...
Orchard, Elizabeth Duncan (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2010-02)Primary producers play a critical role in the oceanic food chain and the global cycling of carbon. The marine diazotroph Trichodesmium is a major contributor to both primary production and nitrogen fixation in the tropical ...
Chappell, Phoebe Dreux (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2009-06)Trichodesmium spp. are considered the dominant nitrogen (N) fixing cyanobacteria in tropical and subtropical oceans, regimes frequently characterized by low iron (Fe). Limited information exists about what levels of Fe ...