Xenopus staufen2 is required for anterior endodermal organ formation
Citable URI
https://hdl.handle.net/1912/5111As published
https://doi.org/10.1002/dvg.22000Keyword
Pancreas; RNA binding protein; Organizer; BMP; MorpholinoAbstract
Defining the regulatory molecular networks involved in patterning the developing anterior endoderm is essential to understanding how the pancreas, liver, stomach and duodenum are discretely specified from each other. In this study, we analyzed the expression and function of the double-stranded RNA-binding protein Staufen2 in Xenopus laevis endoderm. We found that staufen2 was broadly expressed within the developing endoderm beginning at gastrulation becoming localized to the anterior endoderm at later stages. Through morpholino-mediated knockdown, we demonstrate that Staufen2 function is required for proper formation of the stomach, liver and pancreas. We define that its function is required during gastrulation for proper patterning of the dorsal-ventral axis and that it acts to regulate expression of BMP signaling components.
Description
Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of John Wiley & Sons for personal use, not for redistribution. The definitive version was published in genesis 50 (2012): 251-259, doi:10.1002/dvg.22000.
Collections
Suggested Citation
Preprint: Bilogan, Cassandra K., Horb, Marko E., "Xenopus staufen2 is required for anterior endodermal organ formation", 2011-11, https://doi.org/10.1002/dvg.22000, https://hdl.handle.net/1912/5111Related items
Showing items related by title, author, creator and subject.
-
Series 3A: Multiple stressor experiments on T. pseudonana (CCMP1014) - Chlorophyll, particulate organic carbon and particulate organic nitrogen.
Passow, Uta; Laws, Edward; D'Souza, Nigel (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-06-29)The experiments were designed to test the combined effects of CO2, temperatures, and light on growth and photophysiology of the diatom T. pseudonana CCMP1014 in a multifactorial design. This dataset contains measurements ... -
Series 2A-3: Multiple stressor experiments on T. pseudonana (CCMP1014) – Chlorophyll, particulate organic carbon and particulate organic nitrogen
Passow, Uta; Laws, Edward; Sweet, Julia (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30)These experiments were designed to test the combined effects of temperatures and light intensity on the growth growth rate (mu) and photophysiology of the diatom Thalassiosira pseudonana CCMP 1014 in a multifactorial design. ... -
Series 1B-4: Multiple stressor experiments on T. pseudonana (CCMP1335) – Chlorophyll, particulate organic carbon and particulate organic nitrogen
Passow, Uta; Laws, Edward; Sweet, Julia (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: bco-dmo-data@whoi.edu, 2020-11-30)Four follow-up experiments on the combined effect of light and temperature changes on the growth rate (mu) and photophysiology of Thalassiosira pseudonana CCMP 1335 were conducted to supplement / repeat series 1A experiments. ...