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    Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate

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    Author's manuscript (180.5Kb)
    Supplementary data (51.17Kb)
    Date
    2011-07
    Author
    Perry, D. Alexander  Concept link
    Morrison, Hilary G.  Concept link
    Adam, Rodney D.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/5050
    As published
    https://doi.org/10.1016/j.molbiopara.2011.07.008
    Keyword
     Optical map; Genome; Pulsed field gel electrophoresis; Subtelomeric variation 
    Abstract
    The Giardia lamblia genome consists of 12 Mb divided among 5 chromosomes ranging in size from approximately 1 to 4 Mb. The assembled contigs of the genotype A1 isolate, WB, were previously mapped along the 5 chromosomes on the basis of hybridization of plasmid clones representing the contigs to chromosomes separated by PFGE. In the current report, we have generated an MluI optical map of the WB genome to improve the accuracy of the physical map. This has allowed us to correct several assembly errors and to better define the extent of the subtelomeric regions that are not included in the genome assembly.
    Description
    Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Molecular and Biochemical Parasitology 180 (2011): 112-114, doi:10.1016/j.molbiopara.2011.07.008.
    Collections
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    Suggested Citation
    Preprint: Perry, D. Alexander, Morrison, Hilary G., Adam, Rodney D., "Optical map of the genotype A1 WB C6 Giardia lamblia genome isolate", 2011-07, https://doi.org/10.1016/j.molbiopara.2011.07.008, https://hdl.handle.net/1912/5050
     

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