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dc.contributor.authorGadsby, David C.  Concept link
dc.contributor.authorBezanilla, Francisco  Concept link
dc.contributor.authorRakowski, Robert F.  Concept link
dc.contributor.authorDe Weer, Paul  Concept link
dc.contributor.authorHolmgren, Miguel  Concept link
dc.identifier.citationNature Communications 3 (2012): 669en_US
dc.description© Macmillan Publishers Limited, 2012. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Nature Communications 3 (2012): 669, doi:10.1038/ncomms1673.en_US
dc.description.abstractNa+/K+ pumps move net charge through the cell membrane by mediating unequal exchange of intracellular Na+ and extracellular K+. Most charge moves during transitions that release Na+ to the cell exterior. When pumps are constrained to bind and release only Na+, a membrane voltage-step redistributes pumps among conformations with zero, one, two or three bound Na+, thereby transiently generating current. By applying rapid voltage steps to squid giant axons, we previously identified three components in such transient currents, with distinct relaxation speeds: fast (which nearly parallels the voltage-jump time course), medium speed (τm=0.2–0.5 ms) and slow (τs=1–10 ms). Here we show that these three components are tightly correlated, both in their magnitudes and in the time courses of their changes. The correlations reveal the dynamics of the conformational rearrangements that release three Na+ to the exterior (or sequester them into their binding sites) one at a time, in an obligatorily sequential manner.en_US
dc.description.sponsorshipThis research was directly supported by the Intramural Research Program of the National Institutes of Health (NIH), NINDS, grants NIH HL36783 to D.C.G., and NIH U54GM087519 and R01GM030376 to F.B.en_US
dc.publisherNature Publishing Groupen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported*
dc.titleThe dynamic relationships between the three events that release individual Na+ ions from the Na+/K+-ATPaseen_US

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