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dc.contributor.authorWurch, Louie L.  Concept link
dc.contributor.authorBertrand, Erin M.  Concept link
dc.contributor.authorSaito, Mak A.  Concept link
dc.contributor.authorVan Mooy, Benjamin A. S.  Concept link
dc.contributor.authorDyhrman, Sonya T.  Concept link
dc.date.accessioned2012-01-31T14:01:58Z
dc.date.available2012-01-31T14:01:58Z
dc.date.issued2011-12-14
dc.identifier.citationPLoS One 6 (2011): e28949en_US
dc.identifier.urihttps://hdl.handle.net/1912/5008
dc.description© The Author(s), 2011. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in PLoS One 6 (2011): e28949, doi:10.1371/journal.pone.0028949.en_US
dc.description.abstractShotgun mass spectrometry was used to detect proteins in the harmful alga, Aureococcus anophagefferens, and monitor their relative abundance across nutrient replete (control), phosphate-deficient (−P) and −P refed with phosphate (P-refed) conditions. Spectral counting techniques identified differentially abundant proteins and demonstrated that under phosphate deficiency, A. anophagefferens increases proteins involved in both inorganic and organic phosphorus (P) scavenging, including a phosphate transporter, 5′-nucleotidase, and alkaline phosphatase. Additionally, an increase in abundance of a sulfolipid biosynthesis protein was detected in −P and P-refed conditions. Analysis of the polar membrane lipids showed that cellular concentrations of the sulfolipid sulphoquinovosyldiacylglycerol (SQDG) were nearly two-fold greater in the −P condition versus the control condition, while cellular phospholipids were approximately 8-fold less. Transcript and protein abundances were more tightly coupled for gene products involved in P metabolism compared to those involved in a range of other metabolic functions. Comparison of protein abundances between the −P and P-refed conditions identified differences in the timing of protein degradation and turnover. This suggests that culture studies examining nutrient starvation responses will be valuable in interpreting protein abundance patterns for cellular nutritional status and history in metaproteomic datasets.en_US
dc.description.sponsorshipResearch for this work was supported by a National Oceanic and Atmospheric Administration ECOHAB grant (#NA09NOS4780206) and National Science Foundation grant (#OCE-0723667) and a STAR Research Assistance Agreement No. R-83041501-0 awarded by the U.S. Environmental Protection Agency. Further support came from the Woods Hole Coastal Ocean Institute. LLW was supported by a Environmental Protection Agency STAR Fellowship (#FP916901). EMB was supported by a National Science Foundation (NSF) Graduate Research Fellowship (#2007037200) and an Environmental Protection Agency STAR Fellowship (#F6E20324).en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.urihttps://doi.org/10.1371/journal.pone.0028949
dc.rightsAttribution 3.0 Unported*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/*
dc.titleProteome changes driven by phosphorus deficiency and recovery in the brown tide-forming alga Aureococcus anophagefferensen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0028949


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