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    Bcl-xL regulates metabolic efficiency of neurons through interaction with the mitochondrial F1FO ATP synthase

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    Date
    2011-06
    Author
    Alavian, Kambiz N.  Concept link
    Li, Hongmei  Concept link
    Collis, Leon P.  Concept link
    Bonanni, Laura  Concept link
    Zeng, Lu  Concept link
    Sacchetti, Silvio  Concept link
    Lazrove, Emma  Concept link
    Nabili, Panah  Concept link
    Flaherty, Benjamin  Concept link
    Graham, Morven  Concept link
    Chen, Yingbei  Concept link
    Messerli, Shanta M.  Concept link
    Mariggio, Maria A.  Concept link
    Rahner, Christoph  Concept link
    McNay, Ewan  Concept link
    Shore, Gordon  Concept link
    Smith, Peter J. S.  Concept link
    Hardwick, J. Marie  Concept link
    Jonas, Elizabeth A.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/4905
    As published
    https://doi.org/10.1038/ncb2330
    Abstract
    Anti-apoptotic BCL-2 family proteins such as Bcl-xL protect cells from death by sequestering apoptotic molecules, but also contribute to normal neuronal function. We find in hippocampal neurons that Bcl-xL enhances the efficiency of energy metabolism. Our evidence suggests that Bcl-xL interacts directly with the beta subunit of the F1FO ATP synthase, decreasing an ion leak within the F1FO ATPase complex and thereby increasing net transport of H+ by F1FO during F1FO ATPase activity. By patch clamping submitochondrial vesicles enriched in F1FO ATP synthase complexes, we find that, in the presence of ATP, pharmacological or genetic inhibition of Bcl-xL increases the membrane leak conductance. In addition, recombinant Bcl-xL protein directly increases ATPase activity of purified synthase complexes, while inhibition of endogenous Bcl-xL decreases F1FO enzymatic activity. Our findings suggest that increased mitochondrial efficiency contributes to the enhanced synaptic efficacy found in Bcl-xL expressing neurons.
    Description
    Author Posting. © The Author(s), 2011. This is the author's version of the work. It is posted here by permission of Nature Publishing Group for personal use, not for redistribution. The definitive version was published in Nature Cell Biology 13 (2011): 1224–1233, doi:10.1038/ncb2330.
    Collections
    • BioCurrents Research Center
    Suggested Citation
    Preprint: Alavian, Kambiz N., Li, Hongmei, Collis, Leon P., Bonanni, Laura, Zeng, Lu, Sacchetti, Silvio, Lazrove, Emma, Nabili, Panah, Flaherty, Benjamin, Graham, Morven, Chen, Yingbei, Messerli, Shanta M., Mariggio, Maria A., Rahner, Christoph, McNay, Ewan, Shore, Gordon, Smith, Peter J. S., Hardwick, J. Marie, Jonas, Elizabeth A., "Bcl-xL regulates metabolic efficiency of neurons through interaction with the mitochondrial F1FO ATP synthase", 2011-06, https://doi.org/10.1038/ncb2330, https://hdl.handle.net/1912/4905
     
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