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dc.contributor.authorDucklow, Hugh W.  Concept link
dc.contributor.authorMyers, Kristen M. S.  Concept link
dc.contributor.authorErickson, Matthew  Concept link
dc.contributor.authorGhiglione, Jean-Francois  Concept link
dc.contributor.authorMurray, Alison E.  Concept link
dc.date.accessioned2011-11-21T19:41:38Z
dc.date.available2011-11-21T19:41:38Z
dc.date.issued2011-09-20
dc.identifier.citationAquatic Microbial Ecology 64: 205-220en_US
dc.identifier.urihttps://hdl.handle.net/1912/4890
dc.descriptionAuthor Posting. © Inter-Research, 2011. This article is posted here by permission of Inter-Research for personal use, not for redistribution. The definitive version was published in Aquatic Microbial Ecology 64: 205-220, doi:10.3354/ame01519.en_US
dc.description.abstractAlong the western Antarctic Peninsula, marine bacterioplankton respond to the spring phytoplankton bloom with increases in abundance, production and growth rates, and a seasonal succession in bacterial community composition (BCC). We investigated the response of the bacterial community to experimental additions of glucose and ammonium, alone or in combination, incubated in replicate carboys (each: 50 l) over 10 d in November 2006. Changes in bulk properties (abundance, production rates) in the incubations resembled observations in the nearshore environment over 8 seasons (2001 to 2002 through 2008 to 2009) at Palmer Stn (64.8°S, 64.1°W). Changes in bulk properties and BCC in ammonium-amended carboys were small relative to controls, compared to the glucose-amended treatments. The BCC in Day 0 and Day 10 controls and ammonium treatments were >72% similar when assessed by denaturing-gradient gel electrophoresis (DGGE), length heterogeneity polymerase chain reaction (LH-PCR) and capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) fingerprinting techniques. Bacterial abundance increased 2- to 10-fold and leucine incorporation rates increased 2- to 30-fold in the glucose treatments over 6 d. The BCC in carboys receiving glucose (with or without ammonium) remained >60% similar to that in Day 0 controls at 6 d and evolved to <20% similar to that in Day 0 controls after 10 d incubation. The increases in bacterial production rates, and the changes in BCC, suggest that selection for glucose-utilizing bacteria was slow under the ambient environmental conditions. The results suggest that organic carbon enrichment is a major factor influencing the observed winter-to-summer increase in bacterial abundance and activity. In contrast, the BCC was relatively robust, changing little until after repeated additions of glucose and prolonged (~10 d) incubation.en_US
dc.description.sponsorshipH.W.D. and A.E.M. were supported by US NSF grants ANT-0632278 and ANT- 0632389, respectively. This research was partly supported by NSF OPP-0217282 (Palmer LTER). J.F.G. was supported by the Institut Français pour la Recherche et la Technologie Polaires (IFRTP).en_US
dc.format.mimetypeapplication/pdf
dc.language.isoenen_US
dc.publisherInter-Researchen_US
dc.relation.urihttps://doi.org/10.3354/ame01519
dc.subjectAntarcticaen_US
dc.subjectBacterial community compositionen_US
dc.subjectBioassayen_US
dc.subjectMarine bacterioplanktonen_US
dc.titleResponse of a summertime Antarctic marine ­bacterial community to glucose and ammonium enrichmenten_US
dc.typeArticleen_US
dc.identifier.doi10.3354/ame01519


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