Recent Submissions

  • Transgenic Xenopus laevis line for in vivo labeling of nephrons within the kidney 

    Corkins, Mark E.; Hanania, Hannah L.; Krneta-Stankic, Vanja; DeLay, Bridget D.; Pearl, Esther J.; Lee, Moonsup; Ji, Hong; Davidson, Alan J.; Horb, Marko E.; Miller, Rachel K. (MDPI AG, 2018-04-06)
    Xenopus laevis embryos are an established model for studying kidney development. The nephron structure and genetic pathways that regulate nephrogenesis are conserved between Xenopus and humans, allowing for the study of ...
  • Tidal and diel orchestration of behaviour and gene expression in an intertidal mollusc 

    Schnytzer, Yisrael; Simon-Blecher, Noa; Li, J.; Ben-Asher, H. Waldman; Salmon-Divon, Mali; Achituv, Yair; Hughes, Michael E.; Levy, Oren (Nature Publishing Group, 2018-03-20)
    Intertidal inhabitants are exposed to the 24-hour solar day, and the 12.4 hour rising and falling of the tides. One or both of these cycles govern intertidal organisms’ behaviour and physiology, yet little is known about ...
  • Microvillar and ciliary defects in zebrafish lacking an actin-binding bioactive peptide amidating enzyme 

    Kumar, Dhivya; Thomason, Rebecca T.; Yankova, Maya; Gitlin, Jonathan D.; Mains, Richard E.; Eipper, Betty A.; King, Stephen M. (Nature Publishing Group, 2018-03-14)
    The assembly of membranous extensions such as microvilli and cilia in polarized cells is a tightly regulated, yet poorly understood, process. Peptidylglycine α-amidating monooxygenase (PAM), a membrane enzyme essential for ...
  • Targeted knockout of lhx1 via CRISPR/Cas9 gene editing in the Xenopus laevis kidney 

    DeLay, Bridget D.; Corkins, Mark E.; Hanania, Hannah L.; Salanga, Matthew C.; Deng, Jian Min; Sudou, Norihiro; Taira, Masanori; Horb, Marko E.; Miller, Rachel K. (2017-09-29)
    Studying genes involved in organogenesis is often difficult because many of these genes are also essential for early development. The allotetraploid frog, Xenopus laevis, is commonly used to study developmental processes, ...
  • Cell lineage and cell cycling analyses of the 4d micromere using live imaging in the marine annelid Platynereis dumerilii 

    Ozpolat, B. Duygu; Handberg-Thorsager, Mette; Vervoort, Michel; Balavoine, Guillaume (eLife, 2017-12-12)
    Cell lineage, cell cycle, and cell fate are tightly associated in developmental processes, but in vivo studies at single-cell resolution showing the intricacies of these associations are rare due to technical limitations. ...
  • Highly conserved molecular pathways, including Wnt signaling, promote functional recovery from spinal cord injury in lampreys 

    Herman, Paige E.; Papatheodorou, Angelos; Bryant, Stephanie A.; Waterbury, Courtney K. M.; Herdy, Joseph R.; Arcese, Anthony A.; Buxbaum, Joseph D.; Smith, Jeramiah J.; Morgan, Jennifer R.; Bloom, Ona E. (Nature Publishing Group, 2018-01-15)
    In mammals, spinal cord injury (SCI) leads to dramatic losses in neurons and synaptic connections, and consequently function. Unlike mammals, lampreys are vertebrates that undergo spontaneous regeneration and achieve ...
  • Density imaging of heterochromatin in live cells using orientation-independent-DIC microscopy 

    Imai, Ryosuke; Nozaki, Tadasu; Tani, Tomomi; Kaizu, Kazunari; Hibino, Kayo; Ide, Satoru; Tamura, Sachiko; Takahashi, Koichi; Shribak, Michael; Maeshima, Kazuhiro (American Society for Cell Biology, 2017-08-23)
    In eukaryotic cells, highly condensed inactive/silenced chromatin has long been called “heterochromatin.” However, recent research suggests that such regions are in fact not fully transcriptionally silent and that there ...
  • α-Synuclein dimers impair vesicle fission during clathrin-mediated synaptic vesicle recycling 

    Medeiros, Audrey T.; Soll, Lindsey G.; Tessari, Isabella; Bubacco, Luigi; Morgan, Jennifer R. (Frontiers Media, 2017-12-11)
    α-Synuclein is a presynaptic protein that regulates synaptic vesicle (SV) trafficking. In Parkinson’s disease (PD) and several other neurodegenerative disorders, aberrant oligomerization and aggregation of α-synuclein lead ...
  • Single-fluorophore orientation determination with multiview polarized illumination : modeling and microscope design 

    Chandler, Talon; Mehta, Shalin B.; Shroff, Hari; Oldenbourg, Rudolf; La Riviere, Patrick J. (Optical Society, 2017-12-01)
    We investigate the use of polarized illumination in multiview microscopes for determining the orientation of single-molecule fluorescence transition dipoles. First, we relate the orientation of single dipoles to measurable ...
  • Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration 

    Nordenfelt, Pontus; Moore, Travis I.; Mehta, Shalin B.; Kalappurakkal, Joseph Mathew; Swaminathan, Vinay; Koga, Nobuyasu; Lambert, Talley J.; Baker, David; Waters, Jennifer C.; Oldenbourg, Rudolf; Tani, Tomomi; Mayor, Satyajit; Waterman, Clare M.; Springer, Timothy (Nature Publishing Group, 2017-12-11)
    Integrin αβ heterodimer cell surface receptors mediate adhesive interactions that provide traction for cell migration. Here, we test whether the integrin, when engaged to an extracellular ligand and the cytoskeleton, adopts ...
  • Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments 

    Spira, Felix; Cuylen-Haering, Sara; Mehta, Shalin B.; Samwer, Matthias; Reversat, Anne; Verma, Amitabh; Oldenbourg, Rudolf; Sixt, Michael; Gerlich, Daniel W. (eLife, 2017-11-06)
    The actomyosin ring generates force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization and the mechanism of contractility is not well understood. We quantified actin filament order ...
  • Comparison of chemical compounds associated with sclerites from healthy and diseased sea fan corals (Gorgonia ventalina) 

    Toledo-Hernández, Carlos; Ruiz-Diaz, Claudia P.; Díaz-Vázquez, Liz M.; Santiago-Cárdenas, Vanessa; Rosario-Berrios, Derick N.; García-Almedina, Derek M.; Roberson, Loretta M. (PeerJ, 2017-08-25)
    The roles of gorgonian sclerites as structural components and predator deterrents have been widely studied. Yet their role as barriers against microbes has only recently been investigated, and even less is known about the ...
  • A-to-I RNA editing in the earliest-diverging Eumetazoan phyla 

    Porath, Hagit T.; Schaffer, Amos A.; Kaniewska, Paulina; Alon, Shahar; Eisenberg, Eli; Rosenthal, Joshua J. C.; Levanon, Erez; Levy, Oren (Oxford University Press, 2017-04-08)
    The highly conserved ADAR enzymes, found in all multicellular metazoans, catalyze the editing of mRNA transcripts by the deamination of adenosines to inosines. This type of editing has two general outcomes: site specific ...
  • Dynamic organization of chromatin domains revealed by super-resolution live-dell imaging 

    Nozaki, Tadasu; Imai, Ryosuke; Tanbo, Mai; Nagashima, Ryosuke; Tamura, Sachiko; Tani, Tomomi; Joti, Yasumasa; Tomita, Masaru; Hibino, Kayo; Kanemaki, Masato T.; Wendt, Kerstin S. (2017-06)
    The eukaryotic genome is organized within cells as chromatin. For proper information output, higher-order chromatin structures can be regulated dynamically. How such structures form and behave in various cellular processes ...
  • Expanding the genetic toolkit in Xenopus : approaches and opportunities for human disease modeling 

    Tandon, Panna; Conlon, Frank; Furlow, J. David; Horb, Marko E. (Elsevier, 2016-04-22)
    The amphibian model Xenopus, has been used extensively over the past century to study multiple aspects of cell and developmental biology. Xenopus offers advantages of a non-mammalian system, including high fecundity, ...
  • Characterization of trace metal content in the developing zebrafish embryo 

    Thomason, Rebecca T.; Pettiglio, Michael A.; Herrera, Carolina; Kao, Clara; Gitlin, Jonathan D.; Bartnikas, Thomas B. (Public Library of Science, 2017-06-15)
    Trace metals are essential for health but toxic when present in excess. The maintenance of trace metals at physiologic levels reflects both import and export by cells and absorption and excretion by organs. The mechanism ...
  • Luteinizing Hormone is an effective replacement for hCG to induce ovulation in Xenopus 

    Wlizla, Marcin; Falco, Rosalia; Peshkin, Leonid; Parlow, Albert F.; Horb, Marko E. (Elsevier, 2016-06-02)
    Injection of human Chorionic Gonadotropin (hCG) directly into the dorsal lymph sac of Xenopus is a commonly used protocol for induction of ovulation, but recent shortages in the stocks of commercially available hCG as well ...
  • Generation of a Xenopus laevis F1 albino J strain by genome editing and oocyte host-transfer 

    Ratzan, Wil; Falco, Rosalia; Salanga, Cristy; Salanga, Matthew C.; Horb, Marko E. (Elsevier, 2016-03-15)
    Completion of the Xenopus laevis genome sequence from inbred J strain animals has facilitated the generation of germline mutant X. laevis using targeted genome editing. In the last few years, numerous reports have demonstrated ...
  • Trade-off between transcriptome plasticity and genome evolution in cephalopods 

    Liscovitch-Brauer, Noa; Alon, Shahar; Porath, Hagit T.; Elstein, Boaz; Unger, Ron; Ziv, Tamar; Admon, Arie; Levanon, Erez; Rosenthal, Joshua J. C.; Eisenberg, Eli (2017-03)
    RNA editing, a post-transcriptional process, allows the diversification of proteomes beyond the genomic blueprint; however it is infrequently used among animals. Recent reports suggesting increased levels of RNA editing ...
  • Changeable camouflage : how well can flounder resemble the colour and spatial scale of substrates in their natural habitats? 

    Akkaynak, Derya; Siemann, Liese A.; Barbosa, Alexandra; Mathger, Lydia M. (Royal Society, 2017-03-08)
    Flounder change colour and pattern for camouflage. We used a spectrometer to measure reflectance spectra and a digital camera to capture body patterns of two flounder species camouflaged on four natural backgrounds of ...

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