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Ancient DNA techniques : applications for deep-water corals

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dc.contributor.author Waller, Rhian G.
dc.contributor.author Adkins, Jess F.
dc.contributor.author Robinson, Laura F.
dc.contributor.author Shank, Timothy M.
dc.date.accessioned 2011-08-18T20:30:11Z
dc.date.available 2011-08-18T20:30:11Z
dc.date.issued 2007-11-01
dc.identifier.citation Bulletin of Marine Science 81 (2007): 351-359 en_US
dc.identifier.uri http://hdl.handle.net/1912/4764
dc.description Author Posting. © University of Miami - Rosenstiel School of Marine and Atmospheric Science, 2007. This article is posted here by permission of University of Miami - Rosenstiel School of Marine and Atmospheric Science for personal use, not for redistribution. The definitive version was published in Bulletin of Marine Science 81 (2007): 351-359. en_US
dc.description.abstract The potential applications of ancient DNA (aDNA) techniques have been realized relatively recently, and have been revolutionized by the advent of pCR techniques in the mid 1980s. Although these techniques have been proven valuable in ancient specimens of up to 100,000 yrs old, their use in the marine realm has been largely limited to mammals and fish. Using modifications of techniques developed for skeletons of whales and mammals, we have produced a method for extracting and amplifying aDNA from sub-fossil (not embedded in rock) deep-water corals that has been successful in yielding 351 base pairs of the ITS2 region in sub-fossil Desmophyllum dianthus (Esper, 1794) and Lophelia pertusa (Linnaeus, 1758). The comparison of DNA sequences from fossil and live specimens resulted in clustering by species, demonstrating the validity of this new aDNA method. Sub-fossil scler-actinian corals are readily dated using U-series techniques, and so the abundance of directly-dateable skeletons in the world's oceans, provides an extremely useful archive for investigating the interactions of environmental pressures (in particular ocean circulation, climate change) on the past distribution, and the evolution of deep-water corals across the globe. en_US
dc.description.sponsorship Support for this project was provided by National Science Foundation grants OCE 0096373 (JFA), OCE 0095331 (Daniel Scheirer, USGS), OCE 0136871 [D. Yoerger (WH OI) and (TMS)], OCE 0624627 (TMS and RGW) and NOAA’s Office of Exploration grant NA05OAR4601054 (TMS, RGW, and JFA). We are also grateful for the enabling support of the Ocean Life Institute and the Ocean and Climate Change Institute of the Woods Hole Oceanographic Institution en_US
dc.format.mimetype application/pdf
dc.language.iso en_US en_US
dc.publisher University of Miami - Rosenstiel School of Marine and Atmospheric Science en_US
dc.relation.uri http://www.ingentaconnect.com/content/umrsmas/bullmar/2007/00000081/00000003/art00005
dc.title Ancient DNA techniques : applications for deep-water corals en_US
dc.type Article en_US


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