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dc.contributor.authorCox, Alysia D.  Concept link
dc.coverage.spatialCosta Rica Upwelling dome
dc.date.accessioned2011-06-14T13:41:43Z
dc.date.available2011-06-14T13:41:43Z
dc.date.issued2011-06
dc.identifier.urihttps://hdl.handle.net/1912/4645
dc.descriptionSubmitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution June 2011en_US
dc.description.abstractA combination of uptake field studies on natural phytoplankton assemblages and laboratory proteomic and physiological experiments on cyanobacterial isolates were conducted investigating the interactions of cadmium (Cd), zinc (Zn), and phosphorus (P) in marine Synechococcus. Enriched stable isotope field uptake studies of 110Cd in the Costa Rica Upwelling dome, a Synechococcus feature, showed that uptake of Cd occurs in waters shallower than 40 m, correlates positively with chlorophyll a concentrations and is roughly equivalent to the calculated upwelling flux of cadmium inside the dome. In laboratory experiments, Synechococcus WH5701 cells exposed to low picomolar quantities of free Cd under Zn deficiency show similar growth rates to no added Cd treatments during exponential growth phase, but show differences in relative abundances of many proteins involved in carbon and sulfur metabolism suggesting a great metabolic impact. During stationary phase, chronic Cd exposure in this coastal isolate causes an increase in relative chlorophyll a fluorescence and faster mortality rates. The interactions of acute Cd exposure at low picomolar levels with Zn and phosphate (PO4 3-) were investigated in Synechococcus WH8102, an open ocean isolate. The presence of Zn appears vital to the response of the organism to different PO4 3- concentrations. Comparisons with literature transcriptome analyses of PO4 3- stress show similar increases in relative abundance of PO4 3- stress response proteins including a PO4 3- binding protein and a Zn-requiring alkaline phosphatase. A bacterial metallothionein, a Zn-associated protein, appears to be correlated with proteins present under low PO4 3- conditions. Together, these experiments suggest that the interactions of Cd and Zn can affect Synechococcus and play a role in the acquisition of PO4 3-.en_US
dc.description.sponsorshipThe work in this thesis was supported by a MIT Presidential Graduate Fellowship for one year, the Academic Programs Office for one semester, and awards to Mak Saito from the Office of Naval Research for three years. Funding awards to Mak Saito from NSF Chemical Oceanography, the Center for Microbial Oceanography Research and Education, and WHOI Ocean Life Institute also supported this research.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherMassachusetts Institute of Technology and Woods Hole Oceanographic Institutionen_US
dc.relation.ispartofseriesWHOI Thesesen_US
dc.subjectPrimary productivityen_US
dc.subjectBiogeochemistryen_US
dc.subjectKnorr (Ship : 1970-) Cruise KN182-05en_US
dc.titleInteractions of cadmium, zinc, and phosphorus in marine Synechococcus : field uptake, physiological and proteomic studiesen_US
dc.typeThesisen_US
dc.identifier.doi10.1575/1912/4645


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