Life cycle studies of the red tide dinoflagellate species complex Alexandrium tamarense
Brosnahan, Michael L.
MetadataShow full item record
LocationCasco Bay, ME
Blooms of toxic species within the algal dinoflagellate species complex Alexandrium tamarense may cause Paralytic Shellfish Poisoning, a significant and growing environmental threat worldwide. However, blooms of closely related nontoxic A. tamarense also occur, sometimes in close geographical proximity to toxic blooms. This thesis explores the interactions between toxic and nontoxic blooms by examining sexual crosses between each of five ribosomal clades within the A. tamarense complex (termed Groups I-V). Several lines of evidence argue that these clades represent separate species. Particular emphasis was given to interactions between toxic Group I and nontoxic Group III species because they are among the most closely related A. tamarense clades and because they share a natural range boundary in several parts of the world. Interspecies hybridization appeared widespread between different clades and between geographically dispersed isolates. However, subsequent germination studies of hypnozygotes produced from combinations of Group I and Group III clones failed to yield new vegetative cultures in multiple trials. The possibility that these hypnozygotes were actually inbred (i.e. the result of pairs of only Group I or only Group III gametes) was considered and rejected using a nested PCR assay that was developed to assess the parentage of individual cysts. The assay was also suitable for analyzing cysts collected from the field and was applied to individual cysts collected from Belfast Lough, an area where both Group I and Group III blooms were known to occur. Two Group I/Group III hybrids were detected in fourteen successful assays from the Belfast sample, showing that hybridization does occur in nature. These findings have several important implications. First, the failure of Group I/Group III hybrids to produce new vegetative cultures serves as a proof that the A. tamarense clades represent cryptic species because they are unable to produce genetic intermediates. Second, the presence of hybrid cysts in Belfast Lough indicates ongoing displacement of a nontoxic population by a toxic one (or vice versa) in that region. Third, the inviability of toxic/nontoxic hybrids suggests a remediation strategy whereby the recurrence of toxic A. tamarense blooms might be combated through the introduction of nontoxic cells.
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution February 2011
Suggested CitationThesis: Brosnahan, Michael L., "Life cycle studies of the red tide dinoflagellate species complex Alexandrium tamarense", 2011-02, DOI:10.1575/1912/4603, https://hdl.handle.net/1912/4603
Showing items related by title, author, creator and subject.
Lilly, Emily L. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2003-09)The incidence and known distribution of paralytic shellfish poisoning (PSP) have both increased dramatically in recent decades. A concurrent rise in bloom frequency and geographic range of PSP toxin-producing Alexandrium ...
Blooms of the toxic dinoflagellate Alexandrium fundyense in the Gulf of Maine : investigations using physical-biological model Stock, Charles A. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2005-02)Blooms of the toxic dinoflagellate Alexandriumfundyense are annually recurrent in the western Gulf of Maine (WGOM) and pose a serious economic and public health threat. Transitions between and vital rates within the life ...
Franks, Peter J. S. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1990-05)Numerous studies have shown dinoflagellate blooms to be closely related to density discontinuities and fronts in the ocean. The spatial and temporal patterns of the dinoflagellate population depend on the predominant ...