Rapid analysis of 13C in plant-wax n-alkanes for reconstruction of terrestrial vegetation signals from aquatic sediments
McDuffee, Kelsey E.
Eglinton, Timothy I.
Sessions, Alex L.
Sylva, Sean P.
Hayes, John M.
MetadataShow full item record
Long-chain, odd-carbon-numbered C25 to C35 n-alkanes are characteristic components of epicuticular waxes produced by terrestrial higher plants. They are delivered to aquatic systems via eolian and fluvial transport and are preserved in underlying sediments. The isotopic compositions of these products can serve as records of past vegetation. We have developed a rapid method for stable carbon isotopic analyses of total plant-wax n-alkanes using a novel, moving-wire system coupled to an isotope-ratio mass spectrometer (MW-irMS). The n-alkane fractions are prepared from sediment samples by (1) saponification and extraction with organic solvents, (2) chromatographic separation using silica gel, (3) isolation of straight-chain carbon skeletons using a zeolite molecular sieve, and (4) oxidation and removal of unsaturated hydrocarbons with RuO4. Short-chain n-alkanes of nonvascular plant origin (<C25) are removed by evaporation on the moving wire. Test samples processed using this procedure yielded n-alkane fractions essentially free of interfering components. The δ13C values obtained by MW-irMS did not differ significantly from weighted averages of individual n-alkane δ13C values obtained by irmGC-MS. Isotopic variations in compound-class n-alkane fractions from a latitudinal transect of core-top sediments from the Southwest African margin (3°N–28°S) were congruent with those measured by compound-specific isotopic analyses of plant-wax n-alkanes. The amplitude of the variations was smaller, indicating contributions from non-plant-wax hydrocarbons, but the measurements revealed variations in carbon isotopic composition that are consistent with vegetation zones on the adjacent continent.
Author Posting. © American Geophysical Union, 2004. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geochemistry Geophysics Geosystems 5 (2004): Q10004, doi:10.1029/2004GC000772.
Suggested CitationArticle: McDuffee, Kelsey E., Eglinton, Timothy I., Sessions, Alex L., Sylva, Sean P., Wagner, Thomas, Hayes, John M., "Rapid analysis of 13C in plant-wax n-alkanes for reconstruction of terrestrial vegetation signals from aquatic sediments", Geochemistry Geophysics Geosystems 5 (2004): Q10004, DOI:10.1029/2004GC000772, https://hdl.handle.net/1912/457
Showing items related by title, author, creator and subject.
Response of plant nutrient stoichiometry to fertilization varied with plant tissues in a tropical forest Mo, Qifeng; Zou, Bi; Li, Yingwen; Chen, Yao; Zhang, Weixin; Mao, Rong; Ding, Yongzhen; Wang, Jun; Lu, Xiankai; Li, Xiaobo; Tang, Jianwu; Li, Zhian; Wang, Faming (Nature Publishing Group, 2015-09-29)Plant N:P ratios are widely used as indices of nutrient limitation in terrestrial ecosystems, but the response of these metrics in different plant tissues to altered N and P availability and their interactions remains ...
Salguero-Gomez, Roberto; Jones, Owen R.; Archer, C. Ruth; Buckley, Yvonne M.; Che-Castaldo, Judy; Caswell, Hal; Hodgson, David; Scheuerlein, Alexander; Conde, Dalia A.; Brinks, Erik; de Buhr, Hendrik; Farack, Claudia; Gottschalk, France; Hartmann, Alexander; Henning, Anne; Hoppe, Gabriel; Romer, Gesa; Runge, Jens; Ruoff, Tara; Wille, Julia; Zeh, Stefan; Davison, Raziel; Vieregg, Dirk; Baudisch, Annette; Altwegg, Res; Colchero, Fernando; Dong, Ming; de Kroon, Hans; Lebreton, Jean-Dominique; Metcalf, Charlotte J. E.; Neel, Maile M.; Parker, Ingrid M.; Takada, Takenori; Valverde, Teresa; Velez-Espino, Luis A.; Wardle, Glenda M.; Franco, Miguel; Vaupel, James W. (John Wiley & Sons, 2014-11-09)Schedules of survival, growth and reproduction are key life-history traits. Data on how these traits vary among species and populations are fundamental to our understanding of the ecological conditions that have shaped ...
Cesium-134 and 137 activities in the central North Pacific Ocean after the Fukushima Dai-ichi Nuclear Power Plant accident Kamenik, J.; Dulaiova, Henrieta; Buesseler, Ken O.; Pike, Steven M.; Stastna, K. (Copernicus Publications on behalf of the European Geosciences Union, 2013-09-23)Surface seawater 134Cs and 137Cs samples were collected in the central and western North Pacific Ocean during the 2 yr after the Fukushima Dai-ichi Nuclear Power Plant accident to monitor dispersion patterns of these ...