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dc.contributor.authorPekkurnaz, Gulcin
dc.contributor.authorFera, Andrea
dc.contributor.authorZimmerberg-Helms, Jessica
dc.contributor.authorDeGiorgis, Joseph A.
dc.contributor.authorBezrukov, Ludmila
dc.contributor.authorBlank, Paul S.
dc.contributor.authorMazar, Julia
dc.contributor.authorReese, Thomas S.
dc.contributor.authorZimmerberg, Joshua
dc.date.accessioned2011-05-09T19:15:51Z
dc.date.available2011-05-09T19:15:51Z
dc.date.issued2011-04
dc.identifier.citationBiological Bulletin 220 (2011): 89-96en_US
dc.identifier.urihttp://hdl.handle.net/1912/4555
dc.descriptionAuthor Posting. © Marine Biological Laboratory, 2011. This article is posted here by permission of Marine Biological Laboratory for personal use, not for redistribution. The definitive version was published in Biological Bulletin 220 (2011): 89-96.en_US
dc.description.abstractSynaptic vesicles contain a variety of proteins and lipids that mediate fusion with the pre-synaptic membrane. Although the structures of many synaptic vesicle proteins are known, an overall picture of how they are organized at the vesicle surface is lacking. In this paper, we describe a better method for the isolation of squid synaptic vesicles and characterize the results. For highly pure and intact synaptic vesicles from squid optic lobe, glycerol density gradient centrifugation was the key step. Different electron microscopic methods show that vesicle membrane surfaces are largely covered with structures corresponding to surface proteins. Each vesicle contains several stalked globular structures that extend from the vesicle surface and are consistent with the V-ATPase. BLAST search of a library of squid expressed sequence tags identifies 10 V-ATPase subunits, which are expressed in the squid stellate ganglia. Negative-stain tomography demonstrates directly that vesicles flatten during the drying step of negative staining, and furthermore shows details of individual vesicles and other proteins at the vesicle surface.en_US
dc.description.sponsorshipJAD is supported by the RI-INBRE program award # P20RR016457-10 from the National Center for Research Resources (NCRR), NIH.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherMarine Biological Laboratoryen_US
dc.relation.urihttp://www.biolbull.org/cgi/content/abstract/220/2/89?ct=ct
dc.titleIsolation and ultrastructural characterization of squid synaptic vesiclesen_US
dc.typeArticleen_US


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