Evolution of a major drug metabolizing enzyme defect in the domestic cat and other felidae : phylogenetic timing and the role of hypercarnivory
Figure S3: Phylogenetic trees constructed for UGT1A1 and UGT1A6 exon 1 sequences using three different inference methods (78.51Kb)
Table S1: Genbank IDs of novel and existing UGT1A1 and UGT1A6 exon 1 sequences evaluated in this study (14.95Kb)
Table S2: Classification of species based on observed dietary behavior or inferred from the literature (19.74Kb)
Table S3: Protein content of commercial zoo animal diets formulated for various Carnivora in relation to the dietary classification proposed in this study (7.171Kb)
Reed, J. Michael
Starks, Philip T.
Kaufman, Gretchen E.
Goldstone, Jared V.
Roelke, Melody E.
O'Brien, Stephen J.
Frank, Laurence G.
Court, Michael H.
MetadataShow full item record
The domestic cat (Felis catus) shows remarkable sensitivity to the adverse effects of phenolic drugs, including acetaminophen and aspirin, as well as structurally-related toxicants found in the diet and environment. This idiosyncrasy results from pseudogenization of the gene encoding UDP-glucuronosyltransferase (UGT) 1A6, the major species-conserved phenol detoxification enzyme. Here, we established the phylogenetic timing of disruptive UGT1A6 mutations and explored the hypothesis that gene inactivation in cats was enabled by minimal exposure to plant-derived toxicants. Fixation of the UGT1A6 pseudogene was estimated to have occurred between 35 and 11 million years ago with all extant Felidae having dysfunctional UGT1A6. Out of 22 additional taxa sampled, representative of most Carnivora families, only brown hyena (Parahyaena brunnea) and northern elephant seal (Mirounga angustirostris) showed inactivating UGT1A6 mutations. A comprehensive literature review of the natural diet of the sampled taxa indicated that all species with defective UGT1A6 were hypercarnivores (>70% dietary animal matter). Furthermore those species with UGT1A6 defects showed evidence for reduced amino acid constraint (increased dN/dS ratios approaching the neutral selection value of 1.0) as compared with species with intact UGT1A6. In contrast, there was no evidence for reduced amino acid constraint for these same species within UGT1A1, the gene encoding the enzyme responsible for detoxification of endogenously generated bilirubin. Our results provide the first evidence suggesting that diet may have played a permissive role in the devolution of a mammalian drug metabolizing enzyme. Further work is needed to establish whether these preliminary findings can be generalized to all Carnivora.
This is an open-access article, free of all copyright. The definitive version was published in PLoS One 6(2011): e18046, doi:10.1371/journal.pone.0018046.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Quantity, composition, and source of sediment collected in sediment traps along the fringing coral reef off Molokai, Hawaii Bothner, Michael H.; Reynolds, Richard L.; Casso, Michael A.; Storlazzi, Curt D.; Field, Michael E. (Elsevier B.V., 2006-03-20)Sediment traps were used to evaluate the frequency, cause, and relative intensity of sediment mobility/resuspension along the fringing coral reef off southern Molokai (February 2000–May 2002). Two storms with high rainfall, ...
Murawski, Steven A.; Steele, John H.; Taylor, Phillip; Fogarty, Michael J.; Sissenwine, Michael P.; Ford, Michael; Suchman, Cynthia (Oxford University Press, 2009-08-30)Effective marine ecosystem-based management (EBM) requires understanding the key processes and relationships controlling the aspects of biodiversity, productivity, and resilience to perturbations. Unfortunately, the scales, ...
Live-cell imaging RNAi screen identifies PP2A–B55α and importin-β1 as key mitotic exit regulators in human cells Schmitz, Michael H. A.; Held, Michael; Janssens, Veerle; Hutchins, James R. A.; Hudecz, Otto; Ivanova, Elitsa; Goris, Jozef; Trinkle-Mulcahy, Laura; Lamond, Angus I.; Poser, Ina; Hyman, Anthony A.; Mechtler, Karl; Peters, Jan-Michael; Gerlich, Daniel W. (2010-07)When vertebrate cells exit mitosis, they reorganize various cellular structures to build functional interphase cells1. This depends on Cdk1 inactivation and subsequent dephosphorylation of its substrates2-4. Members of ...