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DGGE-based detection method for Quahog Parasite Unknown (QPX)

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dc.contributor.author Gast, Rebecca J.
dc.contributor.author Cushman, E.
dc.contributor.author Moran, Dawn M.
dc.contributor.author Uhlinger, Kevin R.
dc.contributor.author Leavitt, Dale F.
dc.contributor.author Smolowitz, Roxanna M.
dc.date.accessioned 2011-04-22T15:33:07Z
dc.date.available 2011-04-22T15:33:07Z
dc.date.issued 2006-06-12
dc.identifier.citation Diseases of Aquatic Organisms 70 (2006): 115-122 en_US
dc.identifier.uri http://hdl.handle.net/1912/4499
dc.description Author Posting. © Inter-Research, 2006. This article is posted here by permission of Inter-Research for personal use, not for redistribution. The definitive version was published in Diseases of Aquatic Organisms 70 (2006): 115-122, doi:10.3354/dao070115. en_US
dc.description.abstract Quahog Parasite Unknown (QPX) is a significant cause of hard clam Mercenaria mercenaria mortality along the northeast coast of the United States. It infects both wild and cultured clams, often annually in plots that are heavily farmed. Subclinically infected clams can be identified by histological examination of the mantle tissue, but there is currently no method available to monitor the presence of QPX in the environment. Here, we report on a polymerase chain reaction (PCR)-based method that will facilitate the detection of QPX in natural samples and seed clams. With our method, between 10 and 100 QPX cells can be detected in 1 l of water, 1 g of sediment and 100 mg of clam tissue. Denaturing gradient gel electrophoresis (DGGE) is used to establish whether the PCR products are the same as those in the control QPX culture. We used the method to screen 100 seed clams of 15 mm, and found that 10 to 12% of the clams were positive for the presence of the QPX organism. This method represents a reliable and sensitive procedure for screening both environmental samples and potentially contaminated small clams. en_US
dc.description.sponsorship Quahog Parasite Unknown (QPX) is a significant cause of hard clam Mercenaria mercenaria mortality along the northeast coast of the United States. It infects both wild and cultured clams, often annually in plots that are heavily farmed. Subclinically infected clams can be identified by histological examination of the mantle tissue, but there is currently no method available to monitor the presence of QPX in the environment. Here, we report on a polymerase chain reaction (PCR)-based method that will facilitate the detection of QPX in natural samples and seed clams. With our method, between 10 and 100 QPX cells can be detected in 1 l of water, 1 g of sediment and 100 mg of clam tissue. Denaturing gradient gel electrophoresis (DGGE) is used to establish whether the PCR products are the same as those in the control QPX culture. We used the method to screen 100 seed clams of 15 mm, and found that 10 to 12% of the clams were positive for the presence of the QPX organism. This method represents a reliable and sensitive procedure for screening both environmental samples and potentially contaminated small clams. en_US
dc.format.mimetype application/pdf
dc.language.iso en en_US
dc.publisher Inter-Research en_US
dc.relation.uri http://dx.doi.org/10.3354/dao070115
dc.subject Quahog Parasite Unknown en_US
dc.subject Detection limit en_US
dc.subject Seed clams en_US
dc.subject SSU rDNA en_US
dc.title DGGE-based detection method for Quahog Parasite Unknown (QPX) en_US
dc.type Article en_US
dc.identifier.doi 10.3354/dao070115


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