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dc.contributor.authorGast, Rebecca J.
dc.contributor.authorCushman, E.
dc.contributor.authorMoran, Dawn M.
dc.contributor.authorUhlinger, Kevin R.
dc.contributor.authorLeavitt, Dale F.
dc.contributor.authorSmolowitz, Roxanna M.
dc.date.accessioned2011-04-22T15:33:07Z
dc.date.available2011-04-22T15:33:07Z
dc.date.issued2006-06-12
dc.identifier.citationDiseases of Aquatic Organisms 70 (2006): 115-122en_US
dc.identifier.urihttp://hdl.handle.net/1912/4499
dc.descriptionAuthor Posting. © Inter-Research, 2006. This article is posted here by permission of Inter-Research for personal use, not for redistribution. The definitive version was published in Diseases of Aquatic Organisms 70 (2006): 115-122, doi:10.3354/dao070115.en_US
dc.description.abstractQuahog Parasite Unknown (QPX) is a significant cause of hard clam Mercenaria mercenaria mortality along the northeast coast of the United States. It infects both wild and cultured clams, often annually in plots that are heavily farmed. Subclinically infected clams can be identified by histological examination of the mantle tissue, but there is currently no method available to monitor the presence of QPX in the environment. Here, we report on a polymerase chain reaction (PCR)-based method that will facilitate the detection of QPX in natural samples and seed clams. With our method, between 10 and 100 QPX cells can be detected in 1 l of water, 1 g of sediment and 100 mg of clam tissue. Denaturing gradient gel electrophoresis (DGGE) is used to establish whether the PCR products are the same as those in the control QPX culture. We used the method to screen 100 seed clams of 15 mm, and found that 10 to 12% of the clams were positive for the presence of the QPX organism. This method represents a reliable and sensitive procedure for screening both environmental samples and potentially contaminated small clams.en_US
dc.description.sponsorshipQuahog Parasite Unknown (QPX) is a significant cause of hard clam Mercenaria mercenaria mortality along the northeast coast of the United States. It infects both wild and cultured clams, often annually in plots that are heavily farmed. Subclinically infected clams can be identified by histological examination of the mantle tissue, but there is currently no method available to monitor the presence of QPX in the environment. Here, we report on a polymerase chain reaction (PCR)-based method that will facilitate the detection of QPX in natural samples and seed clams. With our method, between 10 and 100 QPX cells can be detected in 1 l of water, 1 g of sediment and 100 mg of clam tissue. Denaturing gradient gel electrophoresis (DGGE) is used to establish whether the PCR products are the same as those in the control QPX culture. We used the method to screen 100 seed clams of 15 mm, and found that 10 to 12% of the clams were positive for the presence of the QPX organism. This method represents a reliable and sensitive procedure for screening both environmental samples and potentially contaminated small clams.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoenen_US
dc.publisherInter-Researchen_US
dc.relation.urihttps://doi.org/10.3354/dao070115
dc.subjectQuahog Parasite Unknownen_US
dc.subjectDetection limiten_US
dc.subjectSeed clamsen_US
dc.subjectSSU rDNAen_US
dc.titleDGGE-based detection method for Quahog Parasite Unknown (QPX)en_US
dc.typeArticleen_US
dc.identifier.doi10.3354/dao070115


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