DGGE-based detection method for Quahog Parasite Unknown (QPX)
Gast, Rebecca J.
Moran, Dawn M.
Uhlinger, Kevin R.
Leavitt, Dale F.
Smolowitz, Roxanna M.
MetadataShow full item record
Quahog Parasite Unknown (QPX) is a significant cause of hard clam Mercenaria mercenaria mortality along the northeast coast of the United States. It infects both wild and cultured clams, often annually in plots that are heavily farmed. Subclinically infected clams can be identified by histological examination of the mantle tissue, but there is currently no method available to monitor the presence of QPX in the environment. Here, we report on a polymerase chain reaction (PCR)-based method that will facilitate the detection of QPX in natural samples and seed clams. With our method, between 10 and 100 QPX cells can be detected in 1 l of water, 1 g of sediment and 100 mg of clam tissue. Denaturing gradient gel electrophoresis (DGGE) is used to establish whether the PCR products are the same as those in the control QPX culture. We used the method to screen 100 seed clams of 15 mm, and found that 10 to 12% of the clams were positive for the presence of the QPX organism. This method represents a reliable and sensitive procedure for screening both environmental samples and potentially contaminated small clams.
Author Posting. © Inter-Research, 2006. This article is posted here by permission of Inter-Research for personal use, not for redistribution. The definitive version was published in Diseases of Aquatic Organisms 70 (2006): 115-122, doi:10.3354/dao070115.
Showing items related by title, author, creator and subject.
Epizootiology of Quahog Parasite Unknown (QPX) disease in northern quahogs (=hard clams) Mercenaria mercenaria Lyons, M. Maille; Smolowitz, Roxanna M.; Gomez-Chiarri, Marta; Ward, J. Evan (National Shellfisheries Association, 2007-08)The economically important marine bivalve mollusc, Mercenaria mercenaria, (commonly called a northern quahog or hard clam), has endured considerable mortalities caused by a thraustochytrid pathogen called Quahog Parasite ...
Gast, Rebecca J.; Moran, Dawn M.; Audemard, Corinne; Lyons, M. Maille; De Favari, Jacquelin; Reece, Kimberly S.; Leavitt, Dale F.; Smolowitz, Roxanna M. (Inter-Research, 2008-09-24)Quahog Parasite Unknown (QPX) is the cause of mass mortality events of hard clams Mercenaria mercenaria from Virginia, USA, to New Brunswick, Canada. Aquaculture areas in Massachusetts, USA, have been particularly hard ...
Development of a real time quantitative PCR assay for the hard clam pathogen Quahog Parasite Unknown (QPX) Lyons, M. Maille; Smolowitz, Roxanna M.; Dungan, Christopher F.; Roberts, Steven B. (Inter-Research, 2006-09-14)Quahog Parasite Unknown (QPX) is a thraustochytrid pathogen responsible for catastrophic mortalities of the northern quahog (hard clam) Mercenaria mercenaria. A real-time quantitative polymerase chain reaction (qPCR) assay ...