Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition
Morrison, Hilary G.
Kitzman, Jacob O.
Turner, Emily H.
MacKenzie, Alexandra P.
Caruccio, Nicholas C.
MetadataShow full item record
We characterize and extend a highly efficient method for constructing shotgun fragment libraries in which transposase catalyzes in vitro DNA fragmentation and adaptor incorporation simultaneously. We apply this method to sequencing a human genome and find that coverage biases are comparable to those of conventional protocols. We also extend its capabilities by developing protocols for sub-nanogram library construction, exome capture from 50 ng of input DNA, PCR-free and colony PCR library construction, and 96-plex sample indexing.
© The Authors, 2010. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Genome Biology 11 (2010): R119, doi:10.1186/gb-2010-11-12-r119.
Suggested CitationArticle: Adey, Andrew, Morrison, Hilary G., Asan, Xun, Xu, Kitzman, Jacob O., Turner, Emily H., Stackhouse, Bethany, MacKenzie, Alexandra P., Caruccio, Nicholas C., Zhang, Xiuqing, Shendure, Jay, "Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition", Genome Biology 11 (2010): R119, DOI:10.1186/gb-2010-11-12-r119, https://hdl.handle.net/1912/4401
The following license files are associated with this item: