Radiocarbon dating of alkenones from marine sediments : I. Isolation protocol


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dc.contributor.author Ohkouchi, Naohiko
dc.contributor.author Xu, Li
dc.contributor.author Reddy, Christopher M.
dc.contributor.author Montlucon, Daniel B.
dc.contributor.author Eglinton, Timothy I.
dc.date.accessioned 2011-03-09T20:32:16Z
dc.date.available 2011-03-09T20:32:16Z
dc.date.issued 2005
dc.identifier.citation Radiocarbon 47 (2005): 401-412 en_US
dc.identifier.uri http://hdl.handle.net/1912/4390
dc.description Author Posting. © Arizona Board of Regents on behalf of the University of Arizona, 2005. This article is posted here by permission of Dept. of Geosciences, University of Arizona for personal use, not for redistribution. The definitive version was published in Radiocarbon 47 (2005): 401-412. en_US
dc.description.abstract The chemical and isotopic compositions of long-chain (C36–C39) unsaturated ketones (alkenones), a unique class of algal lipids, encode surface ocean properties useful for paleoceanographic reconstruction. Recently, we have sought to extend the utility of alkenones as oceanic tracers through measurement of their radiocarbon contents. Here, we describe a method for isolation of alkenones from sediments as a compound class based on a sequence of wet chemical techniques. The steps involved, which include silica gel column chromatography, urea adduction, and silver nitrate-silica gel column chromatography, exploit various structural attributes of the alkenones. Amounts of purified alkenones estimated by GC/FID measurements were highly correlated with CO2 yields after sample combustion, indicating purities of greater than 90% for samples containing ≥100 μg C. The degree of alkenone unsaturation ( ) also varied minimally through the procedure. We also describe a high-performance liquid chromatography (HPLC) method to isolate individual alkenones for molecular-level structural and isotopic determination. en_US
dc.description.sponsorship This work was funded through grants from the National Science Foundation (OCE-9809624; OCE- 9907129) and the Japan Society for the Promotion of Science. en_US
dc.format.mimetype application/pdf
dc.language.iso en_US en_US
dc.publisher Dept. of Geosciences, University of Arizona en_US
dc.relation.uri https://journals.uair.arizona.edu/index.php/radiocarbon/article/view/2834
dc.title Radiocarbon dating of alkenones from marine sediments : I. Isolation protocol en_US
dc.type Article en_US

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