Show simple item record

dc.contributor.authorOhkouchi, Naohiko
dc.contributor.authorXu, Li
dc.contributor.authorReddy, Christopher M.
dc.contributor.authorMontlucon, Daniel B.
dc.contributor.authorEglinton, Timothy I.
dc.date.accessioned2011-03-09T20:32:16Z
dc.date.available2011-03-09T20:32:16Z
dc.date.issued2005
dc.identifier.citationRadiocarbon 47 (2005): 401-412en_US
dc.identifier.urihttp://hdl.handle.net/1912/4390
dc.descriptionAuthor Posting. © Arizona Board of Regents on behalf of the University of Arizona, 2005. This article is posted here by permission of Dept. of Geosciences, University of Arizona for personal use, not for redistribution. The definitive version was published in Radiocarbon 47 (2005): 401-412.en_US
dc.description.abstractThe chemical and isotopic compositions of long-chain (C36–C39) unsaturated ketones (alkenones), a unique class of algal lipids, encode surface ocean properties useful for paleoceanographic reconstruction. Recently, we have sought to extend the utility of alkenones as oceanic tracers through measurement of their radiocarbon contents. Here, we describe a method for isolation of alkenones from sediments as a compound class based on a sequence of wet chemical techniques. The steps involved, which include silica gel column chromatography, urea adduction, and silver nitrate-silica gel column chromatography, exploit various structural attributes of the alkenones. Amounts of purified alkenones estimated by GC/FID measurements were highly correlated with CO2 yields after sample combustion, indicating purities of greater than 90% for samples containing ≥100 μg C. The degree of alkenone unsaturation ( ) also varied minimally through the procedure. We also describe a high-performance liquid chromatography (HPLC) method to isolate individual alkenones for molecular-level structural and isotopic determination.en_US
dc.description.sponsorshipThis work was funded through grants from the National Science Foundation (OCE-9809624; OCE- 9907129) and the Japan Society for the Promotion of Science.en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherDept. of Geosciences, University of Arizonaen_US
dc.relation.urihttps://journals.uair.arizona.edu/index.php/radiocarbon/article/view/2834
dc.titleRadiocarbon dating of alkenones from marine sediments : I. Isolation protocolen_US
dc.typeArticleen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record