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    Dielectrophoretic tweezer for isolating and manipulating target cells

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    NBT-2010-0010 ms.pdf (649.5Kb)
    Date
    2010-06-03
    Author
    Menachery, Anoop  Concept link
    Graham, David M.  Concept link
    Messerli, Shanta M.  Concept link
    Pethig, Ronald  Concept link
    Smith, Peter J. S.  Concept link
    Metadata
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    Citable URI
    https://hdl.handle.net/1912/4352
    As published
    https://doi.org/10.1049/iet-nbt.2010.0010
    Abstract
    The ability to isolate and accurately position single cells in three dimensions is becoming increasingly important in many areas of biological research. We describe the design, theoretical modeling and testing of a novel dielectrophoretic (DEP) tweezer for picking out and relocating single target cells.. The device is constructed using facilities available in most electrophysiology laboratories, without the requirement of sophisticated and expensive microfabrication technology, and offers improved practical features over previously reported DEP tweezer designs. The DEP tweezer has been tested using transfected HEI 193 human schwannoma cells, with visual identification of the target cells being aided by labeling the incorporated gene product with a green fluorescent protein.
    Description
    Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Institution of Engineering and Technology for personal use, not for redistribution. The definitive version was published in IET Nanobiotechnology 5 (2011): 1-7, doi:10.1049/iet-nbt.2010.0010.
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    • BioCurrents Research Center
    Suggested Citation
    Preprint: Menachery, Anoop, Graham, David M., Messerli, Shanta M., Pethig, Ronald, Smith, Peter J. S., "Dielectrophoretic tweezer for isolating and manipulating target cells", 2010-06-03, https://doi.org/10.1049/iet-nbt.2010.0010, https://hdl.handle.net/1912/4352
     
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