Ecology and population structure of Vibrionaceae in the coastal ocean
Preheim, Sarah P.
MetadataShow full item record
Extensive genetic diversity has been discovered in the microbial world, yet mechanisms that shape and maintain this diversity remain poorly understood. This thesis investigates to what extent populations of the gamma-proteobacterial family, Vibrionaceae, are ecologically specialized by investigating the distribution across a wide range of environmental categories, such as marine invertebrates or particles in the water column. Additionally, it seeks to determine whether in situ population distributions directly result from a competitive advantage over other Vibrio populations. This was investigated by in vitro competition assays on mixtures of native, sterilized particles. Generalist populations were found to dominate the associations with marine invertebrates, consistent with a model of high migration dominated population assembly. A majority of populations occurred broadly within and among the different types of invertebrates sampled, with one population being a near perfect generalist with regard to seasons, host taxa and body regions. High variability across host individuals, consistent with a scenario of stochastic clonal expansion, was especially pronounced in crab and zooplankton samples. Specialization, demonstrated by specific and reproducible association with different particle types in the water column, is more common than specialization within invertebrate hosts. Co-existing Vibrio species show strong preferences for different types of particulate matter in the water column suggesting that competition for limited resources influences their evolution. While populations show different growth profiles on particle derived substrates, relative growth advantages of specialist populations in competition with other Vibrio populations on native particles may not be sufficient to explain observed environmental distributions. Instead, populations may gain an advantage on these particles by colonizing the living plant or zooplankton prior to death and degradation into particulate matter. In summary, although vibrios are known commensals of marine invertebrates, evidence suggests that population structure within animals is fairly weak compared to suspended particles in the water column. This highlights the importance of comparing multiple environmental categories and migration among them to investigate population structure and adaptation.
Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole oceanographic Institution February 2010
Suggested CitationThesis: Preheim, Sarah P., "Ecology and population structure of Vibrionaceae in the coastal ocean", 2010-02, DOI:10.1575/1912/4333, https://hdl.handle.net/1912/4333
Showing items related by title, author, creator and subject.
Chen, Changsheng (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1992-06)This thesis consists of two parts: (I) variability of currents and water properties in late spring in the northern Great South Channel and (II) numerical study of stratified tidal rectification over Georges Bank. In part ...
Cherian, Deepak A. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2016-09)Eddies in the ocean move westwards. Those shed by western boundary currents must then interact with continental shelf-slope topography at the western boundary. The presence of other eddies and mean lows complicates this ...
Galvin, Lawrence F. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1991-08)Undersea technology is on the verge of equipping remotely operated vehicle (ROV) pilots with a three-dimensional (3-D), real-time display incorporating data from a wide variety of sensors including sonar (sound navigation ...