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Construction, Theory, and Practical Considerations for using Self-referencing of Ca2+-Selective Microelectrodes for Monitoring Extracellular Ca2+ Gradients

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dc.contributor.author Messerli, Mark A.
dc.contributor.author Smith, Peter J. S.
dc.date.accessioned 2010-11-19T15:43:49Z
dc.date.available 2010-11-19T15:43:49Z
dc.date.issued 2010-10
dc.identifier.uri http://hdl.handle.net/1912/4098
dc.description Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Methods in Cell Biology 99 (2010): 91-111, doi:10.1016/B978-0-12-374841-6.00004-9. en_US
dc.description.abstract Ca2+ signaling in the extra- and intracellular domains is linked to Ca2+ transport across the plasma membrane. Non-invasive monitoring of these resulting extracellular Ca2+ gradients with self-referencing of Ca2+-selective microelectrodes is used for studying Ca2+ signaling across Kingdoms. The quantitated Ca2+ flux enables comparison with changes to intracellular [Ca2+] measured with other methods and determination of Ca2+ transport stoichiometry. Here we review the construction of Ca2+-selective microelectrodes, their physical characteristics and their use in self-referencing mode to calculate Ca2+ flux. We also discuss potential complications when using them to measure Ca2+ gradients near the boundary layers of single cells and tissues. en_US
dc.description.sponsorship NIH:NCRR grant P41 RR001395 en_US
dc.format.mimetype application/pdf
dc.language.iso en_US en_US
dc.relation.uri http://dx.doi.org/10.1016/B978-0-12-374841-6.00004-9
dc.title Construction, Theory, and Practical Considerations for using Self-referencing of Ca2+-Selective Microelectrodes for Monitoring Extracellular Ca2+ Gradients en_US
dc.type Preprint en_US


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