Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° North using 13C DNA Stable Isotope Probing and Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization
Richberg, Kevin P.
MetadataShow full item record
LocationEast Pacific Rise
KeywordChemoautotrophic bacteria; Hydrothermal vents; Microbiology; Atlantis (Ship : 1996-) Cruise AT15-38
At deep‐sea hydrothermal vents chemolithoautotrophic microbes mediate the transfer of geothermal chemical energy to higher trophic levels. To better understand these underlying processes and the organisms catalyzing them, this research used DNA Stable Isotope Probing (SIP) combined with Catalyzed Activated Reporter Deposition‐Fluorescence in situ Hybridization (CARD‐FISH) to identify the microorganisms chemoautotrophically supporting the food web at a diffuse flow hydrothermal vent. Both anaerobic and aerobic shipboard incubations containing various augmented electron donor and acceptor species showed that Epsilonproteobacteria were the dominant chemoautotrophs with greater than 70% of the cells counted within the first 24 hours. 13C DNA SIP identified unique organisms not previously characterized from low temperature diffuse flow venting: green sulfur bacteria (Chlorobi‐like organisms) possibly utilizing photoautotrophy, aerobic Lutibacter litoralis‐like organisms growing under anaerobic conditions, and Epsilonproteobacterial Thioreductor sp. at temperatures above maximum known tolerances. This research illustrates both the promise and pitfalls of the SIP technique applied to hydrothermal systems, concluding that timing of the incubation experiments is the critical step in eliminating undesired 13C labeling. These results set the stage for a more thorough future examination of diffuse flow microorganisms by presenting interesting questions that second generation experiments could be designed to answer.
Submitted in partial fulfillment of the requirements for the degree of Master of Science at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution September 2010
Suggested CitationThesis: Richberg, Kevin P., "Identification of chemoautotrophic microorganisms from a diffuse flow hydrothermal vent at EPR 9° North using 13C DNA Stable Isotope Probing and Catalyzed Activated Reporter Deposition-Fluorescence in situ Hybridization", 2010-09, DOI:10.1575/1912/4078, https://hdl.handle.net/1912/4078
Showing items related by title, author, creator and subject.
McNichol, Jesse C. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2016-09)Chemoautotrophic ecosystems at deep-sea hydrothermal vents were discovered in 1977, but not until 1995 were free-living autotrophic Epsilonproteobacteria identified as important microbial community members. Because the ...
Single amplified genomes (SAGs) of chemoautotrophs from global deep sea samples (Dark ocean chemoautotrophs project) Stepanauskas, Ramunas (Biological and Chemical Oceanography Data Management Office (BCO-DMO). Contact: firstname.lastname@example.org, 2021-04-01)This dataset includes accession numbers archived at IMG/M (Integrated Microbial Genomes and Microbiome Samples) at the US Dept. of Energy's Joint Genome Institute. Additional information includes the ocean depth, latitude ...
Bemis, Karen G. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1990-05)Estimates of the heat output of hydrothermal vents, identified along the Endeavor and Southern Segments of the Juan de Fuca Ridge, are used to evaluate the total heat flux associated with hydrothermal circulation for ...