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dc.contributor.authorGatlin, Jesse C.  Concept link
dc.contributor.authorMatov, Alexandre  Concept link
dc.contributor.authorDanuser, Gaudenz  Concept link
dc.contributor.authorMitchison, Timothy J.  Concept link
dc.contributor.authorSalmon, Edward D.  Concept link
dc.date.accessioned2010-10-29T16:38:25Z
dc.date.available2010-10-29T16:38:25Z
dc.date.issued2010-02-22
dc.identifier.citationJournal of Cell Biology 188 (2010): 481-489en_US
dc.identifier.urihttp://hdl.handle.net/1912/4028
dc.description© The Authors, 2010. This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. The definitive version was published in Journal of Cell Biology 188 (2010): 481-489, doi:10.1083/jcb.200907110.en_US
dc.description.abstractSeveral recent models for spindle length regulation propose an elastic pole to pole spindle matrix that is sufficiently strong to bear or antagonize forces generated by microtubules and microtubule motors. We tested this hypothesis using microneedles to skewer metaphase spindles in Xenopus laevis egg extracts. Microneedle tips inserted into a spindle just outside the metaphase plate resulted in spindle movement along the interpolar axis at a velocity slightly slower than microtubule poleward flux, bringing the nearest pole toward the needle. Spindle velocity decreased near the pole, which often split apart slowly, eventually letting the spindle move completely off the needle. When two needles were inserted on either side of the metaphase plate and rapidly moved apart, there was minimal spindle deformation until they reached the poles. In contrast, needle separation in the equatorial direction rapidly increased spindle width as constant length spindle fibers pulled the poles together. These observations indicate that an isotropic spindle matrix does not make a significant mechanical contribution to metaphase spindle length determination.en_US
dc.description.sponsorshipThis work was supported by National Institute of General Medicine grants to J.C. Gatlin (F32GM080049) and E.D. Salmon (GM24364). T.J. Mitchison was funded by a grant from the National Cancer Institute (CA078048-09).en_US
dc.format.mimetypevideo/quicktime
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherRockefeller University Pressen_US
dc.relation.urihttp://dx.doi.org/10.1083/jcb.200907110
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/us/*
dc.titleDirectly probing the mechanical properties of the spindle and its matrixen_US
dc.typeArticleen_US
dc.identifier.doi10.1083/jcb.200907110


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