Show simple item record

dc.contributor.authorGodard, Celine A. J.  Concept link
dc.contributor.authorGoldstone, Jared V.  Concept link
dc.contributor.authorSaid, Maya R.  Concept link
dc.contributor.authorDickerson, Richard L.  Concept link
dc.contributor.authorWoodin, Bruce R.  Concept link
dc.contributor.authorStegeman, John J.  Concept link
dc.date.accessioned2006-01-10T16:43:49Z
dc.date.available2006-01-10T16:43:49Z
dc.date.issued2005-03-11
dc.identifier.urihttps://hdl.handle.net/1912/401
dc.descriptionAuthor Posting. © The Authors, 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version was published in Biochemical and Biophysical Research Communications 331 (2005): 1016-1024, doi:10.1016/j.bbrc.2005.03.231.
dc.description.abstractTwo novel CYP1 genes from teleost fish constituting a new subfamily have been cloned. These paralogous sequences are designated CYP1C1 and CYP1C2. Both genes were initially obtained from untreated scup Stenotomus chrysops tissues by RT-PCR and RACE. Scup CYP1C1 and CYP1C2 code for 524 and 525 amino acids, respectively, and share 80-81% identity at the nucleotide and amino acid levels. Orthologues of CYP1C1 and CYP1C2 were identified in genome databases for other fish species, and both CYP1B1 and CYP1C1 were cloned from zebrafish (Danio rerio). Phylogenetic analysis shows that CYP1Cs and CYP1Bs constitute a sister clade to the CYP1As. Analysis of sequence domains likely to have functional significance suggests the two CYP1Cs in scup may have catalytic functions and/or substrate specificity that differ from each other and from those of mammalian CYP1Bs or CYP1As. RT-PCR results indicate that CYP1C1 and CYP1C2 are variously expressed in several scup organs.en
dc.description.sponsorshipThis work was supported by EPA grant R 827102-01-0 and NIH grants 5 P42-ES07381 and ES04696. JVG is supported by a Ruth L. Kirschstein NRSA Fellowship (F32 ES012794).en
dc.format.extent96256 bytes
dc.format.extent288797 bytes
dc.format.mimetypeapplication/msword
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen
dc.relation.urihttps://doi.org/10.1016/j.bbrc.2005.03.231
dc.subjectCytochrome P450en
dc.subjectCYP1Aen
dc.subjectCYP1B1en
dc.subjectCYP1Cen
dc.subjectPAHen
dc.subjectPlanar aromatic hydrocarbonsen
dc.titleThe new vertebrate CYP1C family : cloning of new subfamily members and phylogenetic analysisen
dc.typePreprinten


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record