• Login
    About WHOAS
    View Item 
    •   WHOAS Home
    • Marine Biological Laboratory
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    • View Item
    •   WHOAS Home
    • Marine Biological Laboratory
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of WHOASCommunities & CollectionsBy Issue DateAuthorsTitlesKeywordsThis CollectionBy Issue DateAuthorsTitlesKeywords

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    The transcriptional response to encystation stimuli in Giardia lamblia is restricted to a small set of genes

    Thumbnail
    View/Open
    MBL_copy.pdf (1.260Mb)
    Date
    2010-07
    Author
    Morf, Laura  Concept link
    Spycher, Cornelia  Concept link
    Rehrauer, Hubert  Concept link
    Fournier, Catharine Aquino  Concept link
    Morrison, Hilary G.  Concept link
    Hehl, Adrian B.  Concept link
    Metadata
    Show full item record
    Citable URI
    https://hdl.handle.net/1912/3938
    As published
    https://doi.org/10.1128/EC.00100-10
    Abstract
    The protozoan parasite Giardia lamblia undergoes stage-differentiation in the small intestine of the host to an environmentally resistant and infectious cyst. Encystation involves secretion of an extracellular matrix comprised of cyst wall proteins (CWPs) and a β(1-3)-GalNAc homopolymer. Upon induction of encystation, genes coding for CWPs are switched on, and mRNAs coding for a transcription factor Myb and enzymes involved in cyst wall glycan synthesis are upregulated. Encystation in vitro is triggered by several protocols, which call for changes in bile concentrations or availability of lipids, and elevated pH. However, the conditions for induction are not standardized and we predicted significant protocol-specific side effects. This makes reliable identification of encystation factors difficult. Here, we exploited the possibility to induce encystation with two different protocols, which we show to be equally effective, for a comparative mRNA profile analysis. The standard encystation protocol induced a bipartite transcriptional response with surprisingly minor involvement of stress genes. A comparative analysis revealed a core set of only 18 encystation genes and showed that a majority of genes was indeed upregulated as a side effect of inducing conditions. We also established a Myb binding sequence as a signature motif in encystation promoters, suggesting coordinated regulation of these factors.
    Description
    Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Eukaryotic Cell 9 (2010): 1566-1576, doi:10.1128/EC.00100-10.
    Collections
    • Josephine Bay Paul Center in Comparative Molecular Biology and Evolution
    Suggested Citation
    Preprint: Morf, Laura, Spycher, Cornelia, Rehrauer, Hubert, Fournier, Catharine Aquino, Morrison, Hilary G., Hehl, Adrian B., "The transcriptional response to encystation stimuli in Giardia lamblia is restricted to a small set of genes", 2010-07, https://doi.org/10.1128/EC.00100-10, https://hdl.handle.net/1912/3938
     
    All Items in WHOAS are protected by original copyright, with all rights reserved, unless otherwise indicated. WHOAS also supports the use of the Creative Commons licenses for original content.
    A service of the MBLWHOI Library | About WHOAS
    Contact Us | Send Feedback | Privacy Policy
    Core Trust Logo