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dc.contributor.authorMartin, Jonathan B.  Concept link
dc.contributor.authorBernhard, Joan M.  Concept link
dc.contributor.authorCurtis, Jason H.  Concept link
dc.contributor.authorRathburn, Anthony E.  Concept link
dc.date.accessioned2010-09-02T13:58:11Z
dc.date.available2010-12-11T09:22:01Z
dc.date.issued2010-06-11
dc.identifier.citationPaleoceanography 25 (2010): PA2211en_US
dc.identifier.urihttps://hdl.handle.net/1912/3898
dc.descriptionAuthor Posting. © American Geophysical Union, 2010. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Paleoceanography 25 (2010): PA2211, doi:10.1029/2009PA001846.en_US
dc.description.abstractCarbon isotopes of foraminiferal tests provide a widely used proxy for past oceanographic environmental conditions. This proxy can be calibrated using live specimens, which are reliably identified with observations of cell ultrastructure. Observations of ultrastructures can also be used for studies of biological characteristics such as diet and presence of symbionts. Combining biological and isotopic studies on individual foraminifera could provide novel information, but standard isotopic methods destroy ultrastructures by desiccating specimens and observations of ultrastructure require removal of carbonate tests, preventing isotope measurements. The approach described here preserves cellular ultrastructure during isotopic analyses by keeping the foraminifera in an aqueous buffer (Phosphate Buffered Saline (PBS)). The technique was developed and standardized with 36 aliquots of NBS-19 standard of similar weight to foraminiferal tests (5 to 123 μg). Standard errors ranged from ± 0.06 to ± 0.85‰ and were caused by CO2 contaminants dissolved in the PBS. The technique was used to measure δ13C values of 96 foraminifera, 10 of which do not precipitate carbonate tests. Calcareous foraminiferal tests had corrected carbon isotope ratios of −8.5 to +3.2‰. This new technique allows comparisons of isotopic compositions of tests made by foraminifera known to be alive at the time of collection with their biological characteristics such as prey composition and presence or absence of putative symbionts. The approach may be applied to additional biomineralizing organisms such as planktonic foraminifera, pteropods, corals, and coccolithophores to elucidate certain biological controls on their paleoceanographic proxy signatures.en_US
dc.description.sponsorshipSupport was provided by NSF grants OCE‐0550396 (to J.B.M.), OCE‐0551001 (to J.M.B.), and OCE‐ 0550401 (to A.E.R.).en_US
dc.format.mimetypeapplication/pdf
dc.language.isoen_USen_US
dc.publisherAmerican Geophysical Unionen_US
dc.relation.urihttps://doi.org/10.1029/2009PA001846
dc.subjectForaminiferaen_US
dc.subjectUltrastructureen_US
dc.subjectCarbon isotopesen_US
dc.titleCombined carbonate carbon isotopic and cellular ultrastructural studies of individual benthic foraminifera : method descriptionen_US
dc.typeArticleen_US
dc.identifier.doi10.1029/2009PA001846


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