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dc.contributor.authorPasapera, Ana M.  Concept link
dc.contributor.authorSchneider, Ian C.  Concept link
dc.contributor.authorRericha, Erin  Concept link
dc.contributor.authorSchlaepfer, David D.  Concept link
dc.contributor.authorWaterman, Clare M.  Concept link
dc.identifier.citationJournal of Cell Biology 188 (2010): 877-890en_US
dc.description© The Authors, 2010. This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. The definitive version was published in Journal of Cell Biology 188 (2010): 877-890, doi:10.1083/jcb.200906012.en_US
dc.description.abstractFocal adhesions (FAs) are mechanosensitive adhesion and signaling complexes that grow and change composition in response to myosin II–mediated cytoskeletal tension in a process known as FA maturation. To understand tension-mediated FA maturation, we sought to identify proteins that are recruited to FAs in a myosin II–dependent manner and to examine the mechanism for their myosin II–sensitive FA association. We find that FA recruitment of both the cytoskeletal adapter protein vinculin and the tyrosine kinase FA kinase (FAK) are myosin II and extracellular matrix (ECM) stiffness dependent. Myosin II activity promotes FAK/Src-mediated phosphorylation of paxillin on tyrosines 31 and 118 and vinculin association with paxillin. We show that phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. These results reveal an important role for paxillin in adhesion mechanosensing via myosin II–mediated FAK phosphorylation of paxillin that promotes vinculin FA recruitment to reinforce the cytoskeletal ECM linkage and drive FA maturation.en_US
dc.description.sponsorshipThis work was supported by NHLBI (C.M. Waterman and A.M. Pasapera; and grant HL093156 to D.D. Schlaepfer) and the Burroughs Wellcome Fund (E. Rericha).en_US
dc.publisherRockefeller University Pressen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 United States*
dc.titleMyosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylationen_US

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