Myosin II activity regulates vinculin recruitment to focal adhesions through FAK-mediated paxillin phosphorylation

Date
2010-03-22Author
Pasapera, Ana M.
Concept link
Schneider, Ian C.
Concept link
Rericha, Erin
Concept link
Schlaepfer, David D.
Concept link
Waterman, Clare M.
Concept link
Metadata
Show full item recordCitable URI
https://hdl.handle.net/1912/3865As published
https://doi.org/10.1083/jcb.200906012DOI
10.1083/jcb.200906012Abstract
Focal adhesions (FAs) are mechanosensitive adhesion and signaling complexes that grow and change composition in response to myosin II–mediated cytoskeletal tension in a process known as FA maturation. To understand tension-mediated FA maturation, we sought to identify proteins that are recruited to FAs in a myosin II–dependent manner and to examine the mechanism for their myosin II–sensitive FA association. We find that FA recruitment of both the cytoskeletal adapter protein vinculin and the tyrosine kinase FA kinase (FAK) are myosin II and extracellular matrix (ECM) stiffness dependent. Myosin II activity promotes FAK/Src-mediated phosphorylation of paxillin on tyrosines 31 and 118 and vinculin association with paxillin. We show that phosphomimic mutations of paxillin can specifically induce the recruitment of vinculin to adhesions independent of myosin II activity. These results reveal an important role for paxillin in adhesion mechanosensing via myosin II–mediated FAK phosphorylation of paxillin that promotes vinculin FA recruitment to reinforce the cytoskeletal ECM linkage and drive FA maturation.
Description
© The Authors, 2010. This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. The definitive version was published in Journal of Cell Biology 188 (2010): 877-890, doi:10.1083/jcb.200906012.
Collections
Suggested Citation
Journal of Cell Biology 188 (2010): 877-890The following license files are associated with this item: