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dc.contributor.authorJaqaman, Khuloud  Concept link
dc.contributor.authorKing, Emma M.  Concept link
dc.contributor.authorAmaro, Ana C.  Concept link
dc.contributor.authorWinter, Jennifer R.  Concept link
dc.contributor.authorDorn, Jonas F.  Concept link
dc.contributor.authorElliott, Hunter L.  Concept link
dc.contributor.authorMchedlishvili, Nunu  Concept link
dc.contributor.authorMcClelland, Sarah E.  Concept link
dc.contributor.authorPorter, Iain M.  Concept link
dc.contributor.authorPosch, Markus  Concept link
dc.contributor.authorToso, Alberto  Concept link
dc.contributor.authorDanuser, Gaudenz  Concept link
dc.contributor.authorMcAinsh, Andrew D.  Concept link
dc.contributor.authorMeraldi, Patrick  Concept link
dc.contributor.authorSwedlow, Jason R.  Concept link
dc.identifier.citationJournal of Cell Biology 188 (2010): 665-679en_US
dc.description© The Authors, 2010. This article is distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. The definitive version was published in Journal of Cell Biology 188 (2010): 665-679, doi:10.1083/jcb.200909005.en_US
dc.description.abstractDuring mitosis in most eukaryotic cells, chromosomes align and form a metaphase plate halfway between the spindle poles, about which they exhibit oscillatory movement. These movements are accompanied by changes in the distance between sister kinetochores, commonly referred to as breathing. We developed a live cell imaging assay combined with computational image analysis to quantify the properties and dynamics of sister kinetochores in three dimensions. We show that baseline oscillation and breathing speeds in late prometaphase and metaphase are set by microtubule depolymerases, whereas oscillation and breathing periods depend on the stiffness of the mechanical linkage between sisters. Metaphase plates become thinner as cells progress toward anaphase as a result of reduced oscillation speed at a relatively constant oscillation period. The progressive slowdown of oscillation speed and its coupling to plate thickness depend nonlinearly on the stiffness of the mechanical linkage between sisters. We propose that metaphase plate formation and thinning require tight control of the state of the mechanical linkage between sisters mediated by centromeric chromatin and cohesion.en_US
dc.description.sponsorshipWe thank Nikon (J.R. Swedlow and G. Danuser) and the Laura and Arthur Colwin Endowed Summer Research Fellowship program (J.R. Swedlow, P. Meraldi, and A.D. McAinsh) for financial support. J.R. Swedlow was supported by a Wellcome Trust Senior Research Fellowship (067433). A.C. Amaro, N. Mchedlishvili, and A. Toso are members of the Life Science Zurich Graduate School in Molecular Life Science. P. Meraldi is the recipient of an SNF-Förderungprofessur and a European Young Investigator Award and is supported by a grant from the Swiss National Science Foundation and ETH. Work in the McAinsh laboratory is supported by the Marie Curie Cancer Care. Work in the Danuser laboratory is supported by the National Institutes of Health (R01 GM68956). K. Jaqaman was supported in part by a Paul Sigler/Agouron postdoctoral fellowship from the Helen Hay Whitney Foundation.en_US
dc.publisherRockefeller University Pressen_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported*
dc.titleKinetochore alignment within the metaphase plate is regulated by centromere stiffness and microtubule depolymerasesen_US

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